MC4R基因表达及其与鸡屠宰性状的相关分析  

The Correlation between the Expression of Melanocortin-4 Receptor Gene and the Carcass Traits in Chicken

作　　者：任善茂[1] 陶勇[1] 周俊[1] 高勤学[1] 王金玉[2] 

机构地区：[1]江苏畜牧兽医职业技术学院,江苏泰州225300 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国畜牧兽医》2011年第1期119-122,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家发改委生物育种专项资助(0231895101);江苏省高新技术项目(BG2004316);江苏省"青蓝工程"基金(200702)

摘　　要：根据鸡MC4R mRNA和看家基因β-actin mRNA序列,分别设计1对引物,以半定量RT-PCR法研究不同品种鸡肾上腺中MC4R基因mRNA表达水平,并分析了其与屠宰性状间的相关关系。结果表明,京海黄鸡公鸡MC4R基因在肾上腺中的表达水平显著高于尤溪麻鸡公鸡(P<0.05);京海黄鸡公鸡的胴体重、胸肌重、腿肌重与MC4R基因在肾上腺中表达水平存在极显著相关(P<0.01);尤溪麻鸡公鸡的胴体重与MC4R基因在肾上腺中表达水平存在极显著相关(P<0.01),胸肌重、腿肌重与MC4R基因在肾上腺中表达水平存在显著相关(P<0.05)。A method of semi-quantity RT-PCR was established to evaluate gene expression of MC4R in adrenal of chicken.The primers of β-actin and MC4R were designed according to their sequence reported.The correlation were analyzed between the expression and the carcass traits.The results showed that the level of the expression of MC4R in male Jinghai yellow chicken was higher significantly than that in male Youxima chicken（P0.05）.The level of the expression of MC4R was very significant with carcass weight,breast muscle weight and leg muscle weight in male Jinghai yellow chicken（P0.01）.The level of the expression of MC4R was very significant with carcass weight in male Youxima chicken（P0.01）,and was significant with breast muscle weight and leg muscle weight in male Youxima chicken（P0.05）.

关 键 词：鸡 MC4R基因 基因表达 屠宰性状 

分 类 号：Q78[生物学—分子生物学]