广西片形吸虫中间宿主—小土窝螺线粒体rrnL基因遗传多态性研究  

DNA Polymorphism in the Partial Mitochondrial rrnL Gene among Galba pervia(the Intermediate Host of Fasiola)Isolates from Guangxi

作　　者：王树艳[1,2] 李鑫[2] 李娟[2] 沈前程[1] 朱兴全[3] 黄维义[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530005 [2]华南农业大学兽医学院,广东广州510642 [3]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046

出　　处：《中国畜牧兽医》2011年第1期165-170,共6页China Animal Husbandry & Veterinary Medicine

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)

摘　　要：为研究广西小土窝螺不同地理株线粒体大亚基(large subunit ribosomal rRNA,rrnL)基因的遗传多态性,对广西大片吸虫6个流行地区96个小土窝螺样品的rrnL基因进行PCR扩增,通过单链构象多态性(single strand conformation poly-morphism,SSCP)技术筛选出具有代表性的样品进行克隆、测序,并用DNAStar 5.0及MEGA 4.0软件加以比对分析。结果显示,6个地区小土窝螺rrnL基因PCR扩增长度约500 bp,在长度为273 bp的序列中检测到15个变异位点,占核苷酸总数的5.49%。百色、南宁和桂林3个地区相同地理株间存在较大的遗传差异。种系发育分析表明,线粒体rrnL基因在一定程度上不是研究小土窝螺种群遗传变异的良好分子标记。本研究为阐明片形吸虫中间宿主—小土窝螺的种群遗传关系提供了基础数据。In order to analyze sequence variation in the partial mitochondrial large subunit ribosomal rRNA gene（rrnL） of Galba pervia from different geographical locations in Guangxi,rrnL genes were amplified from G.pervia isolates obtained from 6 endemic origins.After screening by SSCP technique,representative samples with variable banding patterns were selected for sequencing.DNAStar 5.0 and MEGA 4.0 were used to analyze the sequences subsequently.The results showed that a fragment of the rrnL approximately 500 bp in length was amplified by PCR and a total of 15 variable sites（5.49%） were detected within a 273 bp sequence in 6 endemic origins.There was a notable sequence variation among isolates from same geographical origin in Baise,Nanning and Guilin.Phylogenetic analysis revealed that mitochondrial rrnL was not a good molecular marker for population genetic study of G.pervia to some extent.These results provided novel data for elucidating the genetic variation of G.pervia,the intermediate host of Fasciola in China.

关 键 词：小土窝螺(Galba pervia) 线粒体rrnL基因 PCR-SSCP 序列分析 

分 类 号：Q78[生物学—分子生物学]