不同免疫增效剂对鸡新城疫免疫效果的影响  

Effect on Immune Efficiency of Newcastle Disease with Different Immune Intensifiers

作　　者：李富桂[1] 张文生[2] 梁庆欣[1] 赵丽敏[1] 王志龙[1] 

机构地区：[1]天津农学院动物科学系,天津300384 [2]天津职业大学生物与环境工程学院,天津300402

出　　处：《中国畜牧兽医》2011年第1期188-190,共3页China Animal Husbandry & Veterinary Medicine

基　　金：天津市农业科技成果转化与推广项目<禽呼吸道病的快速诊断和综合防治技术的熟化与推广>(0701100)

摘　　要：为研究鸡转移因子、黄芪、左旋咪唑、灵芝4种免疫增效剂的功效,以鸡新城疫（Newcastle disease,ND）血凝抑制抗体效价作为指标,比较其免疫效果。试验鸡先采血测定ND抗体效价,接种注射ND-IB-EDS灭活油乳剂疫苗,再将试验鸡随机分成5组,1-4组为试验组,给予不同的免疫增效剂,5组为空白对照组。在给药后第4、6、8、10、12、14、16、18、20、22和24天分别采血,采用微量血凝抑制试验检测ND抗体效价,统计后比较其抗体效价的变化。结果表明：灵芝组抗体效价在12 d开始显著高于对照组和其他试验组（P〈0.05）,且保持较高的水平;转移因子组和黄芪组抗体效价从14 d开始均显著高于对照组和左旋咪唑组（P〈0.05）,并分别从第22和20天开始持续在较高水平;左旋咪唑组的抗体效价高于对照组,但未出现显著性差异。由此可见,不同的免疫增效剂对鸡ND疫苗的免疫增强作用各异。综合考虑,临床应用时首选鸡转移因子,其次黄芪,灵芝和左旋咪唑需酌情使用。Immune effects of 4 different immune intensifiers（chicken transfer factor,astragalus,levamisole and ganoderma）were compared in this paper using Newcastle disease（ND） antibody titre as indicator.Blood samples were collected for detecting ND HI titre before experiment,ND-EDS-IB attenuated oil-emulsion vaccine was injected into each bird.And then all chickens were divided into five groups,group 1 to 4 were experimental groups and group 5 was control,and all birds were given with different immune intensifiers.Then,antibody titres of blood samples collected on 4th,6th,8th,10th,12th,14th,16th,18th,20th,22th,24th day post-immunization were detected by HI test,changes of HI titres were received by mathematical statistics.The results showed that HI titre of ganoderma group were significant higher than control group and other groups at 12th day firstly,and kept in high level;there were more distinct enhancement respectively in transfer factor group and astragalus group in 14th day,keeping in high level;the titre of levamisole group was higher than control group after 20th day,but there was no significant differentiation.Therefore,there were various immune efficiencies to ND vaccine with different immune intensifiers.By comprehensive consideration,chicken transfer factor（priority） and astragalus（secondary choice） would be applied in clinic,and ganoderma and levamisole should be used appropriately.

关 键 词：免疫增效剂 免疫效果 新城疫抗体效价 鸡转移因子 黄芪 左旋咪唑 灵芝 

分 类 号：S852.4[农业科学—基础兽医学]