猪繁殖与呼吸综合征病毒变异野毒株与JXA1-R疫苗毒株快速鉴别方法的建立  

Establishment of Distinguishing Method on Detecting Porcine Reproductive and Respiratory Syndrome Virus JXA1 and JXA1-R Strain

作　　者：何玲[1] 裴仉福[1] 

机构地区：[1]广东大华农动物保健品股份有限公司,广东新兴527400

出　　处：《中国畜牧兽医》2011年第1期198-201,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：根据猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)变异野毒JXA1的基因变异序列和疫苗株JXA1-R的基因遗传标志设计合成了2对特异性引物,结合快速RNA抽提试剂和一步法RT-PCR,建立了JXA1变异野毒与JXA1-R疫苗毒的快速鉴别体系,其目的片段大小分别为4002、90 bp,该方法对猪瘟病毒、猪细小病毒、猪圆环病毒2型、猪伪狂犬病病毒的PCR检测结果均为阴性;对JXA1变异野毒与JXA1-R疫苗毒RNA的最低检测量分别为103、104拷贝/μL。该方法敏感、特异,适用于临床样品的检测。Based on the variant sequences of PRRSV JXA1 strain and the genetic markers of JXA1-R strain,two pairs of primers were designed and synthesized,and RT-PCR methods were established for detecting the two viruses with specific gene fragments of 400 bp and 290 bp.There was no cross-reactions with those clinically relevant pathogens,such as classical swine fever virus,porcine pseudorabies virus,porcine circovirus type 2,porcine parvovirus.The sensitivity tests showed that the assay could detect 103 copies/μL RNA of JXA1 and 104 copies/μL RNA of JXA1-R.The RT-PCR established in this study,with high specificity and sensitivity,was very suit for clinic-sample detection.

关 键 词：PRRSV变异株 JXA1-R RT-PCR 

分 类 号：S852.65[农业科学—基础兽医学]