高氧暴露对早产儿血单个核细胞血红素加氧酶-1蛋白表达水平及活性的影响  

The effection on the expression and activity of heme oxygenase-1 in the peripheral blood mononuclear cell of hyperoxia-exposed premature infant

作　　者：全裕凤[1] 郑明慈[1] 欧维林[1] 张华[1] 张培林[1] 

机构地区：[1]广西桂林医学院附属医院新生儿科,541001

出　　处：《中国新生儿科杂志》2011年第1期37-39,共3页Chinese Journal of Neonatology

基　　金：广西科技厅科技攻关基金(0472002-35);广西卫生厅医药卫生基金(Z2001061)

摘　　要：目的观察高氧暴露早产儿血单个核细胞血红素加氧酶-1(HO-1)蛋白表达水平及活性变化。方法选择2008年2月至2009年8月本科住院吸入高浓度氧的40例早产儿(高氧组)和吸入常压空气的40例早产儿(对照组),用Western Blot法检测血中单个核细胞HO-1蛋白表达水平、血清胆红素水平和血浆一氧化碳血红蛋白(COHb)百分比,并进行比较分析。结果与对照组相比,高氧组HO-1蛋白表达明显增加[(1.78±0.25)比(1.12±0.31),P<0.01],胆红素生成量(nmol.mg-1.h-1)及血浆COHb含量(μmol/L)均显著增加[(76.25±11.33)比(53.47±8.73),(6.73±0.42)比(2.47±0.34),P均<0.01]。结论高氧暴露可上调早产儿血单个核细胞HO-1的表达及活性。Objective To investigate the expression and activity of heme oxygenase-1 in the peripheral blood mononuclear cell （PBMC） of hyperoxia-exposed premature infant. Methods The expression of HO-1 protein,the active of HO and COHb in the PBMC of 40 cases three-day-old hyperoxia-exposed （O2 40%） preterm infant （hyperoxia group） and 40 cases air-exposed preterm infant （air group） were measured by western blot. Results Comparing with that of room air group,the expression of HO-1,the activity of HO （nmol·mg-1·h-1） and COHb （μmol/L） in hyperoxia group was significantly increased [（1.78±0.25） vs （1.12±0.31）,（76.25±11.33） vs （53.47±8.73）,（6.73±0.42） vs （2.47±0.34）,P〈0.01]. Conclusions Hyperoxia-exposure increase the expression of HO-1 in the PBMC of hyperoxia-exposed premature infant,maybe due to HO-1 associates with the hyperoxia-induced lung injury of premature infant.

关 键 词：血红素加氧酶-1 高浓度氧 婴儿 早产 

分 类 号：R722.6[医药卫生—儿科]