益气养阴活血利水法对兔视网膜脱离后玻璃体腔液中ET-1表达的影响  

Effects of the Method of Qi-benefitting Yin-Fostering and Blood-invigorating and Diuresis-activating on Expression of ET-1 in Liquor of Vitreous Cavity After Retinal Detachment of Rabbit

作　　者：刘娉[1,2] 彭俊[3] 彭清华[1] 姚小磊[1] 谭涵宇[1] 

机构地区：[1]湖南中医药大学第一附属医院眼科学重点学科,湖南长沙410007 [2]湖南旺旺医院眼科中心,湖南长沙410016 [3]南华大学医学院,湖南衡阳421001

出　　处：《中华中医药学刊》2011年第1期97-100,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助项目(06JJ50056);湖南省教育厅科研基金资助课题(10A094;98B078;06A052);湖南省教育厅中医五官科学重点学科建设项目;国家中医药管理局中医眼科学重点学科建设项目

摘　　要：目的:研究益气养阴活血利水之复明片对兔实验性视网膜脱离后玻璃体腔内ET-1表达的影响。方法:用有色家兔72只设正常对照组(A组),及制作视网膜脱离模型后分模型组(B组)、西药对照组(C组)、复明片组(D组)。并于造模后第7天、14天、21天分别取玻璃体腔液用放射免疫分析方法测定ET-1的表达。结果:视网膜脱离后玻璃体腔中ET-1表达均明显上升,7天时表达骤然升高,对照组明显高于复明片治疗组,且相互间各组比较差异均有统计学意义(P<0.05);14天时结果显示各组表达较7天时明显下调,但仍明显高于正常对照组,西药组与正常对照组、复明片治疗组与正常对照组及复明片治疗组与西药对照组比较差异无统计学意义,其余各组相互间比较差异均有统计学意义(P<0.05);21天时视网膜基本已复位,表达进一步下调,但仍高于正常,且相互间各组比较差异均有统计学意义(P<0.05)。结论:益气养阴活血利水之复明片能下调视网膜脱离后玻璃体腔中ET-1的表达。Objective： To evaluate effects of FuMing Tablets of qi-benefitting,yin-fostering,and blood-invigorating and diuresis-activating on expression of ET-1 in liquor of vitreous cavity of rabbit after retinal detachment.Method： 72 rabbits were divided into normal contrast group（group A）,retinal detachment model group（group B）,western medicine contrast group（group C）,FMT group（group D）.Each group had 9 rabbits.Group B,C,D were made retinal detachment model.After models were made one day they were given medicine.Group A and B were administrated intragastrically with 5mL/kg warm water,once a day.Group C was administrated intragastrically with 5mL/kg western medicine solution,once a day.Group D was administrated intragastrically with 5mL/kg FMT suspension,once a day.After model were made to check the expression of ET-1 in liquor of vitreous cavity on the 7th,14th,and 21st day by radioactive immune analysis.Result： there is an obviously increase of expression of ET-1 in liquor of vitreous cavity of rabbit after retinal detachment.On the 7th day,there is a sharp increase,and the normal contrast group（A group） is much higher than retinal detachment model group（B group）,and the dates of every group make sense in statistics（P〈0.05）;On the 14th day,the results show that a evidently decrease merged comparing to the 7th day,but still higher the normal contrast group（group A）,it doesn’t make sense in statistics to compare western medicine contrast group（group C） with normal contrast group（group A）,normal contrast group（group A） with FuMing Tablets group（group D）,and FuMing Tablets group（group D）,normal contrast group（group A）,with western medicine contrast group（group C）,while it does to compare other groups（P〈0.05）;On the 21st day the retina almost have recovered,the decrease continues,but still higher than normal and the comparison between groups make sense in statistics（P〈0.05）.Conclusion： FMT can decrease expression of ET-1 in liquor of vitreous

关 键 词：益气养阴活血利水法 复明片 视网膜脱离模型 ET-1 兔 

分 类 号：R285.5[医药卫生—中药学]