仔猪渗出性皮炎病原的分离鉴定及耐药性分析  

Isolation,Identification and Antimicrobial Susceptibilities of Pathogens of Exudative Epidermitis

作　　者：张玉玉[1] 吴家强[1] 郭立辉[1] 王兴东 安利国[3] 王金宝[1,3] 

机构地区：[1]山东省农业科学院畜牧兽医研究所和山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [2]寿光市圣城畜牧兽医管理站,山东潍坊262700 [3]山东师范大学生命科学学院,山东济南250014

出　　处：《济南大学学报（自然科学版）》2011年第3期269-273,共5页Journal of University of Jinan(Science and Technology)

基　　金：国家转基因生物新品种培育科技重大专项(2009ZX08009-143B);山东省中青年科学家科研奖励基金(2009BSC02016);山东省留学回国人员科技活动择优资助项目(鲁人社函[2009]341号)

摘　　要：为消除渗出性皮炎对仔猪的危害,对发生该病的病死仔猪进行细菌分离培养。通过革兰氏染色、生化试验与16SrDNA基因序列同源性分析鉴定:分离出的两株细菌为猪葡萄球菌和松鼠葡萄球菌。猪葡萄球菌和松鼠葡萄球菌能分别产生表皮脱落毒素ExhB和ExhC,表明这两株葡萄球菌都有致病力。猪葡萄球菌和松鼠葡萄球菌均对头孢菌素Ⅰ类(先锋霉素Ⅰ、Ⅴ、Ⅵ)、头孢噻肟和丁胺卡那敏感,在临床上可选择这些药物预防和治疗该病。To eliminate harm of exudative epidermitis（EE） to piglets,pathogens of EE were isolated and studied.Two gram positive staphylococcus strains were isolated from sick piglets suffered EE.The two isolates were identified as staphylococcus hyicus and staphylococcus sciuri by biochemistry test and analyzing the sequence of 16S rDNA gene.S.hyicus can produce exfoliative toxin ExhB,while S.sciuri can produce exfoliative toxin ExhC,which indicates the two isolates are pathogenic.The two isolates are sensitive to cefalotin,cephazolin,cephradine,cefotaxime and amikacin which shows sick piglets can be treated with these antibiotics.

关 键 词：仔猪渗出性皮炎 猪葡萄球菌 松鼠葡萄球菌 表皮脱落毒素 

分 类 号：S858.28[农业科学—临床兽医学]