正交试验法优选莱菔子水溶性生物碱提取工艺  

Optimization of Extraction Technology of Water-soluble Semen Raphani Alkaloids by Orthogonal Design

作　　者：丁臻[1] 蔡梅超[1] 周洪雷[1] 查慧敏[2] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省警官培训学院,山东济南250014

出　　处：《食品与药品》2011年第1期19-21,共3页Food and Drug

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB512601);山东省科技发展计划项目(2008GG2NS02011);山东省中药泰山学者岗位研究团队项目

摘　　要：目的优选莱菔子水溶性生物碱的提取工艺。方法以80%醇浸出物得率和莱菔子水溶性生物碱的雷氏盐沉淀量为考察指标,对加水量、提取次数、提取时间3个因素进行正交试验。结果通过正交试验优化确定的提取条件为:药材以10倍水提取3次,每次1.5 h。结论优选确定的工艺可用于提取莱菔子水溶性生物碱。Objective To optimize the extraction technology of water-soluble Semen Raphani alkaloids, Methods The orthogonal design was adopted with the yield of 80% alcohol extract and Reinecke salt precipitation amount of watersoluble Semen Raphani alkaloids as the indices. Three factors were studied in this experiment, including the amount of water, extraction times, and time of extraction. Results The optimal extraction technology was to extract the medical material with 10 times water for 3 times, each time for 1.5h. Conclusion This technology can be used for the extraction of water-soluble Semen Raphani alkaloids.

关 键 词：菜菔子 水溶性生物碱 提取 

分 类 号：R282.71[医药卫生—中药学]