DA受体对运动疲劳后纹状体神经元信号转导调节作用的研究  

Regulation Role of Dopamine Receptor on Striatum Neuron after Exercise-induced Fatigue

作　　者：侯莉娟[1] 刘晓莉[1] 乔德才[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875

出　　处：《西安体育学院学报》2011年第1期79-82,共4页Journal of Xi'an Physical Education University

基　　金：国家自然科学基金项目(30971416);北京市自然科学基金项目(5072024)

摘　　要：目的通过观察多巴胺D1受体(Dopamine D1Receptors,D1DR)和多巴胺D2受体(Dopamine D2Receptors,D2DR)拮抗剂对运动疲劳后纹状体神经元电活动的影响,揭示DA系统对运动疲劳后纹状体腹外侧和背外侧神经元电活动的调节作用机制。方法 10天递增负荷游泳运动建立大鼠运动疲劳动物模型。采用玻璃微电极胞外记录技术,观察右脑室(A:0 mm,L:1.6 mm,H:3.4 mm)微量注射DA受体拮抗剂SCH23390和Spiperone l0μL前、后神经元电活动的变化。结果 (1)对照组有28.57%的神经元受到SCH23390的影响,其中使神经元自发放电频率加快兴奋性增加的占16.67%(7/42),兴奋性降低的占11.90%(5/42);SCH23390的诱发作用有一定的潜伏期,且能诱发单放电神经元产生爆发式放电;(2)Spiperone记录中,56.10%的神经元兴奋性受影响,兴奋性增加的占9.76%(4/41),降低的占46.34%(19/41)。Spiperone对实验组放电神经元产生抑制作用的比例显著高于兴奋作用的神经元(P(0.05)。结论运动疲劳后SCH23390可诱发神经元单放电向爆发式放电的转变,Spiperone对纹状体神经元的抑制作用加强。Objective After the observation of the effect given by dopamine receptor antagonist on ventrolateral and dorsolateral striatum neuron electrical activity in the wake of exercise - induced fatigue, and it is revealed that there is regulation mechanism of dopamine system. Methods load - increasing swimming exercise for ten days for establishing exercise - induced fatigue model. During the experiment , the steady firing was recorded with glass mieroelectrode, SCH23390 and Spiperone （10 μL） were injected to the right ventricle （A ：0 mm, L：1. 6 mm, H：3.4 mm）and then to investigate the firing chan-es. Results 1 ） About 28.57% of neurons affected by SCH23390 in the control group, Among them there were 16.67% （7/42） neuron excitability increased, while 11.90% （5/42）decreased. There was certain latent period in SCH23390＇ s regulation role, and SCH23390 can induce single firing to bursting firing. 2） About 56.10% of neurons affected by Spiperone, Among them there were 9.76% （4/41） neuron excitability increased, while 46.34% （ 19/41 ） decreased. In experimental group, the effect of Spiperone on the discharge of neurons, the inhibitory effects were significantly higher than the excitatory effects （ P 〈 0.05 ）. Conclusion After exercise - induced fatigue, SCH23390 can induce the change from single firing to bursting firing. Spiperone can enhance the inhibitory effects on striatum neurons.

关 键 词：运动疲劳 纹状体 微量注射 受体拮抗剂 神经元电活动 

分 类 号：G804.7[文化科学—运动人体科学]