大鼠下丘脑SST及垂体SSTR-1对6周递增负荷运动的应答和适应  

Responsive and Adaptive Changes of Hypothalamus SST and Pituitary SSTR-1 of Rat Brought by Incremental Exercise for 6 Weeks

作　　者：高新友[1] 刘军[1] 郝选明[2] 武小路[3] 

机构地区：[1]西安体育学院健康科学系,西安710068 [2]华南师范大学体育科学学院,广州510631 [3]陕西理工学院体育学院理论教研室,陕西汉中723000

出　　处：《西安体育学院学报》2011年第1期87-93,共7页Journal of Xi'an Physical Education University

基　　金：陕西省自然科学基础研究计划项目(2007C258)

摘　　要：目的观察递增负荷运动造成免疫功能下降过程中生长抑素(SST)和生长抑素1型受体(SSTR-1)的应答和适应变化特征,探讨其可能机理。方法 120只SD雄性大鼠随机分为试验组和对照组,又依据取样时间的不同分为15小组,建立递增负荷训练模型。分别于第0、2、4、6周末的运动前安静状态、运动后即刻和运动后3 h,将大鼠全身灌流固定后取脑及垂体,冠状位连续石蜡切片后免疫组织化学染色,所得图像使用Image-Pro Plus6.0图像分析软件分析。结果 (1)下丘脑中SST主要分布在3V旁室周核的上半部分,为胞质阳性;SSTR-1主要分布在垂体边缘,为胞膜阳性。(2)各组大鼠下丘脑SST及垂体SSTR-1对6周递增负荷运动的适应性变化特征为:①对照组大鼠SST在6周内无显著性差异,SSTR-1在第2周时最低,依次为第0、4周,至第6周时最高;②2周安静状态SST较0周明显增多,4周安静时下降至略低于0周,第6周则下降更低;SSTR-1安静状态下4周最高,依次为第6、2和0周。(3)各周大鼠下丘脑SST及垂体SSTR-1对1次性运动的应答性变化特征为:①第0、4周运动后即刻SST的表达增多,而第2、6周减少;训练第0、2、4周大鼠运动后3 h均可恢复至运动前安静状态,第6周则不能恢复;②SSTR-1在运动即刻及恢复3 h后的变化规律与SST一致,但运动结束后3 h的恢复程度较SST低,有滞后现象。结论 (1)在长期递增负荷运动影响下,安静状态时大鼠下丘脑SST运动初期一过性增加,之后呈下降趋势,适宜运动可以减少安静状态SST的表达,有利于维持自身的免疫机能;(2)大鼠垂体SSTR-1对1次性运动应答性反应的规律与SST一致,即接受适宜运动训练的个体运动后即刻表达增多,处于运动应激动员和衰竭阶段的个体运动后即刻表达减少;(3)运动后即刻及安静状态下,过度训练均引起大鼠下丘脑SST及垂体SSTR-1表达减少,对于对抗免疫抑制的发展具有重要意义,是机�Objective Through observing the responsive and adaptive characteristics of hypothalamus SST and pituitary SSTR - 1 to incremental exercise of 6 weeks in rat, this research is try to explores the possible mechanism. Methods 120 SD rats were randomly divided into 2 groups, namely the experimental group and the control group. According to different time of sampling, the 2 groups were further divided into 15 groups again. Then, the 6 weeks of incremental trained model was established. The sample of rat＇ s brain and pituitary were dawn out after perfusion fixation in the wake of respectively 0, 2, 4 and 6 weeks under the condition of quiet situation before exercise, immediate after exercise and 3 hours after exercise respectively. And the samples were stained with immunohistochemical after consecutive coronal paraffin section. The images were analyzed and processed by Image - Pro Plus 6.0 software. Results （ 1 ） SST was mainly distributed in the top half of periventricular nucleus cytoplasm next to 3V in the hypothalamus, and intracellular fluid showed positive; while SSTR - 1 were mainly found in pituitary edge membrane whose cell membrane showed positive. （2）The adaptive changing characteristics of SST in hypothalamus and SSTR - 1 in pituitary in each group rats to incremental exercise of 6 - week with incremental load were as follows ： ①SST of rats in the controlled group has no significant difference during 6 weeks, SSTR - 1 was least in the 2nd weeks, followed by the before training and the 4th weeks, and was maximum in the sixth weeks; ②for quiet state, SST of 2 weeks increased significantly compared with that of 0 weeks, 4 weeks slightly lower than 0 weeks, and 6 weeks more lower ; SSTR - 1 has maximum level in 4 weeks, followed by 6, 2 and 0 weeks. （3） In each week, the responsive changing characteristics of SST in hypothalamus and SSTR - 1 in pituitary of rats to one single exercise were as follows ： ① the expression of SST increased in 0 and 4 week immediately after the exercis

关 键 词：大鼠 递增负荷运动 生长抑素(SST) 生长抑素受体(SSTR) 

分 类 号：G804.7[文化科学—运动人体科学]