苦参素诱导人胃癌MGC-803细胞凋亡的体内外实验研究  

Experimental Study on Oxymatrine Inducing Apoptosis of Human Carcinoma Cells MGC-803 in Vivo and in Vitro

作　　者：朱艳琴[1] 刘粉霞[2] 徐玉芳[1] 

机构地区：[1]河南中医学院基础医学院,郑州450008 [2]河南中医学院第二附属医院,郑州450008

出　　处：《中国药学杂志》2011年第1期28-30,共3页Chinese Pharmaceutical Journal

基　　金：河南省重点科技攻关项目(082102310013)

摘　　要：目的探讨苦参素体内外诱导人胃癌MGC-803细胞凋亡的作用及机制。方法运用流式细胞术检测细胞凋亡率及凋亡相关基因蛋白Bcl-2和Bax的表达水平;运用TUNEL法观察裸鼠皮下移植瘤细胞凋亡情况。结果经体内外实验研究结果表明,苦参素能促进人胃癌MGC-803细胞凋亡,其细胞凋亡率与阴性对照组相比差异具有统计学意义(P(0.01);Bcl-2表达量明显减少,Bax表达量明显增加,差异均具有统计学意义(P(0.05)。结论苦参素体内外均能诱导人胃癌MGC-803细胞凋亡,其机制与上调Bax蛋白表达和下调Bcl-2蛋白表达有关。OBJECTIVE To explore effect of oxymatrine on MGC-803 cell apoptosis and its possible mechanism in vivo and in vitro.METHODS Apoptosis rate and the expression level of Bcl-2 and Bax were determined with flow cytometry.Subcutaneous transplantation tumor cell apoptosis was observed in nude mouse with TUNEL.RESULTS The study in vivo and in vitro indicated that oxymatrine promoted MGC-803 cell apoptosis.The index of apoptosis was significantly different（P﹤0.05） compared with the control group.The expression of Bcl-2 was reduced and the expression of Bax was increased remarkably（P﹤0.05）.CONCLUSION Oxymatrine can induce MGC-803 cell apoptosis in vivo and in vitro.The mechanism was related to up-regulating expression of Bax and down-regulating expression of Bcl-2.

关 键 词：苦参素 流式细胞术 TUNEL 人胃癌MGC-803细胞 凋亡 

分 类 号：R965[医药卫生—药理学]