HPLC测定柴黄片中柴胡皂苷b_1、b_2的含量  

Determination of Saikosaponin b_1 and b_2 in Chai-Huang Tablet by HPLC

作　　者：李军[1] 姜华[1] 张延萍[1] 王新胜[1] 李晓明[1] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471003

出　　处：《中国药学杂志》2011年第1期61-63,共3页Chinese Pharmaceutical Journal

基　　金：河南科技大学青年基金项目(2008QN016);河南科技大学博士科研启动项目(No2007-09001242)

摘　　要：目的建立柴黄片制剂中柴胡皂苷b1、b2的HPLC含量测定方法。方法采用Hypersil C18色谱柱(4.6mm×250 mm,5μm);流动相:甲醇-水(75∶25);流速:1.0 mL·min-1;检测波长:254nm;柱温:30℃。结果柴胡皂苷b1的线性范围为0.015～0.120μg(r=0.999 5),柴胡皂苷b2的线性范围为0.057～0.456μg(r=0.999 8),柴胡皂苷b1、b2的平均加样回收率分别为97.2%、96.9%,RSD分别为2.0%、1.6%。结论本方法简便、可靠、重现性好,可用于柴黄片中柴胡皂苷b1、b2的含量测定,为完善含柴胡类成方制剂的质量评价体系提供参考。OBJECTIVE To establish a HPLC for the quantitative determination of saikosaponin b1 and b2 in Chai-huang Tablet.METHODS The HPLC method was performed on a Hypersil C18 column（4.6 mm×250 mm,5 μm） with MeOH-Water（75∶25） as the mobile phase.The flow rate was 1.0 mL·min^-1.The UV detection wavelength was 254 nm,and the column temperature was set at 30 ℃.RESULTS The calibration curves showed good linear response ranged from 0.015 to 0.120 μg（r=0.999 5） for saikosaponin b1 and from 0.057 to 0.456 μg（r=0.999 8） for saikosaponin b2.The average recoveries were 97.2%（RSD=2.0%） and 96.9%（RSD=1.6%） respectively.CONCLUSION The method is simple,reliable and repeatable,and can be used for the quantitative determination of saikosaponin b1 and b2 in Chai-huang Tablet.

关 键 词：柴黄片 柴胡皂苷 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]