淋菌耐药表型与耐药基因的分析  

Drug resistant phenotype of neisseria gonorrhoeae and drug resistant genes

作　　者：孙各琴[1,2] 张炽伦[1] 王前[2] 张秀明[1] 兰海丽[1] 郑磊[2] 卢兰芬[1] 吴秀娟[1] 

机构地区：[1]中山大学附属中山市人民医院检验医学中心,广东中山528400 [2]南方医科大学,广东中山528400

出　　处：《中华医院感染学杂志》2011年第2期218-220,共3页Chinese Journal of Nosocomiology

基　　金：中山市科技计划项目(20091A034)

摘　　要：目的探讨淋菌多药耐药与耐药基因的关系。方法应用K-B法检测2009年1月-2010年8月分离的40株淋菌对抗菌药物的敏感性,煮沸法提取菌株DNA,随机抽取增其中22株PCR扩TEM、tetM、erm和mefA基因。结果淋菌的敏感性测定显示,多药耐药现象明显;TEM耐药基因的检出率为77.3%,tetM耐药基因的检出率为77.3%,未检出erm和mefA基因。结论淋菌的多药耐药与各耐药基因型之间密切相关。OBJECTIVE To investigate the relationship between the multi-drug resistance and the resistant genes of Neisseria gonorrhoeae.METHODS The drug susceptibilities of 40 strains of N.gonorrhoeae to antibiotics were tested by K-B and agar dilution methods.Total DNA was extracted by boiling methods.Totally 22 strains of N.gonorrhoeae were randomly selected,which were used as the template in PCR to amplify the resistant genes of TEM,tetM,erm and mefA.RESULTS The susceptibilities tests showed that the multi-drug resistance of N.gonorrhoeae was obvious.The detection rates of TEM and tetM were 77.3% and 77.3%,respectively,while erm and mefA were not detected.CONCLUSION It has a close relationship between the multi-drug resistance and the phenotype of resistant genes of N.gonorrhoeae.

关 键 词：淋菌 耐药性 耐药基因 

分 类 号：R378[医药卫生—病原生物学]