幽门螺杆菌感染性胃炎患者胃黏膜组织CagA表达与其培养液中IL-6、IL-8及CRP水平的变化  

Expression of CagA in gastric epithelial tissue and changes of IL-6,IL-8 and CRP levels in tissue culture media in patients with helicobacter pylori infectious gastritis

作　　者：朱宝[1] 袁航[1] 奚惠芳[1] 王俊超[1] 

机构地区：[1]江苏省无锡市人民医院核医学科,江苏无锡214023

出　　处：《中华医院感染学杂志》2011年第2期236-238,共3页Chinese Journal of Nosocomiology

摘　　要：目的研究幽门螺杆菌(Hp)感染相关性胃炎胃黏膜组织细胞毒素相关蛋白A(CagA)表达,3种炎性因子水平的变化及关系。方法选体检中心50名健康人为对照组,消化科52例慢性浅表性胃炎患者为胃炎组,两组胃黏膜组织CagA采用Western Blotting方法检测;胃黏膜组织培养液IL-6、IL-8含量采用放射免疫法测定;C-反应蛋白(CRP)含量采用免疫比浊法检测;Hp测定采用13C-UBT检查和血清抗Hp IgG检测。结果胃炎组与对照组Hp的阳性感染率分别为90.38%和76.00%,差异无统计学意义,但CagA+的表达率则前者(78.85%)显著高于后者组(22.00%)(P<0.05),CagA+/Hp比值胃炎组(87.23%)也明显高于对照组(28.95%)(P<0.05);胃黏膜组织培养液中,IL-6水平对照组、CagA-胃炎组、CagA+胃炎组分别为(136.12±62.27)、(140.26±53.14)、(199.15±129.51)μg/L;对照组与CagA-组差异无统计学意义,CagA+组则显著高于对照组和CagA-组;IL-8水平3组分别为(0.09±0.03)、(0.12±0.08)、(0.45±0.10)μg/L,两两比较,差异有统计学意义(P<0.05);CRP水平则显示CagA-组(2.90±3.51)mg/L与对照组(2.75±2.92)mg/L比较差异无统计学意义,而CagA+组表达水平(8.70±7.31)mg/L则显著高于CagA-组和对照组,均P<0.01。结论 CagA+的表达及胃黏膜组织培养液IL-8水平的变化表明其均参与了慢性胃炎的发病及损害过程,IL-6及CRP水平显示参与度较小。OBJECTIVE To study the expression of gastric epithelial Tissue CagA,changes of IL-6,IL-8 and CRP levels in tissue culture media and their relationships in patients with helicobacter pylori infectious gastritis.METHODS A total of 102 people were included in this study（52 patients and 50 healthy people）.Gastric epithelial tissue CagA was detected by Western Blotting,IL-6 and IL-8 in gastric epithelial tissue culture media was tested by radioimmunoassay and immunnoturbiodimetry,CRP was determined by immunoturbidimetry,and HP was tested by 13C-UBT and serum anti-HPIgG test.The data was processed and analyzed.RESULTS The postive infection rates of HP in gastritis group and the control group were 90.38% and 76.00%,respectively,but there was no significant difference between them（P〈0.05）.However,CagA＋HP infection rate was significantly higher in gastritis group（78.85%） than that in the control group（22.00%,（P〈0.05）,also was the ratio of CagA＋/HP（P〈0.05）.IL-6 level in the control group,CagA-group and CagA＋ group were（136.12±62.27）μg/L,（140.26±53.14）μg/L and（199.15±129.51）μg/L,respectively.There was no significant difference of IL-6 between the CagA-group and the control group,but there was significant difference in CagA＋ group and control group.IL-8 level in the three groups were（0.09±0.03）μg/L,（0.12±0.08）μg/L and（0.45±0.10）μg/L respectively,either CagA＋ group or CagA-group was higher than the control group,but it was more significant in CagA＋ group（P〈0.01）.The development of CRP was similar to IL-6.CONCLUSION CagA＋HP and IL-8 plays an important role in causing inflammation reaction and pathologic damage in the process of gastritis,but IL-6 and CRP take a little part in it.

关 键 词：幽门螺杆菌 胃炎 细胞毒素相关蛋白A 白细胞介素-6 白细胞介素-8 C反应蛋白 

分 类 号：R378[医药卫生—病原生物学]