手性固定相正相高效液相色谱法拆分普萘洛尔对映体  

Resolution of Propranolol Enantiomers by Chiral Solid-Station Phase and NP-HPLC

作　　者：赵静[1] 邹卫[1] 赵华[1] 靳红卫[1] 

机构地区：[1]重庆医科大学药物工程研究中心,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2011年第1期7-11,共5页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立了手性固定相正相高效液相色谱拆分普萘洛尔对映体的方法,考察普萘洛尔对映体在4种多糖类手性色谱柱(Chiralpak AD柱、Chiralcel OD柱、Cniralcel OJ柱和Chiralpak AS柱)(Daicel,250mm×4.6mm,10μm)上的分离行为,同时考察了流动相中醇类以及碱性添加剂二乙胺(DEA)对普萘洛尔对映体分离行为的影响。结果表明,普萘洛尔对映体在Chiralpak AD柱、Chiralcel OD柱以及Cniralcel OJ柱上能够达到基线分离,出峰顺序均是R-(+)-普萘洛尔先出峰。在Chiralpak AS柱上无分离。The resolution of propranolol enantiomers was established by normal-phaseHPLC using chiral solid-station phase.The influences of four polysaccharide chiral columns（Chiralpak AD,Chiralcel OD,Cniralcel OJ,Chiralpak AS）（Daicel,250mm×4.6mm,10μm）,alcohol modifier as well as alkaline modifier diethylamine on propranolol were studied.It was found that propranolol enantiomers were achieved baseline separation on Chiralpak AD,Chiralcel OD and Chiralcel OJ columns,and R-（＋）-propranolol was always first eluted,but there was no serparation on Cniralpak AS column.

关 键 词：手性固定相 正相高效液相色谱法 普萘洛尔对映体 

分 类 号：O657.72[理学—分析化学]