珙桐热休克蛋白序列分析及功能预测  

Sequence Analysis and Structural Prediction on Heat Shock Protein from Davidia Involucrata Baill. Seed

作　　者：刘美[1,2] 苏智先[2] 齐刚[1] 胡进耀[2] 阮期平[2] 

机构地区：[1]西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川省南充市637002 [2]四川省生态学与环境保护技术重点实验室

出　　处：《光谱实验室》2011年第1期36-40,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(30670209);四川省教育厅重点资助项目(09ZX009)

摘　　要：从本实验室已构建好的珙桐种子cDNA文库中获得热休克蛋白18(HSP18)核苷酸序列,该序列编码蛋白属小热休克蛋白(sHSP)。用生物信息学的方法对HSP18从同源性、氨基酸组成、理化性质、疏水性/亲水性、信号肽、跨膜结构域、卷曲螺旋、蛋白质二级及三级结构进行预测和分析。结果表明,珙桐HSP18是不具有信号肽、没有跨膜卷曲螺旋结构的亲水性稳定蛋白,无规则卷曲是其蛋白质结构的主要结构元件,α-螺旋与β-折叠分散于整个多肽链中,并存在1个结构功能域。Heat shock protein 18（HSP18） was one member of small heat shock protein families which was obtained from Davidia involucrata Baill.seed cDNA club in the laboratory.The characteristics of structure and function were analyzed and predicated from the homology,composition of amino acids,physic-chemical characters,hydrophobicity or hydrophilicity,signal peptide,transmembrane structure,coils,secondary and tertiary structure of protein using the bioinformatics tools.The HSP18 in Daridia involucrata Baill.is a hydrophilicity and non-transmembrane protein without signal peptides and coils.The main motifs of its predicted secondary structure is random coil.Alpha helix and beta turn are spread to the whole secondary structure of protein.Its amino acid sequences include one functional domain.

关 键 词：珙桐种子 热休克蛋白18 生物信息学 

分 类 号：Q51[生物学—生物化学]