黑龙江膜荚黄芪多糖及微量元素Fe、Zn、Cu和Mn的测定  

Determination of Polysacchharide and Trace Elements Fe,Zn,Cu and Mn in Astragalus Membranaceus of Heilongjiang

作　　者：李宏岩[1] 周兆平[1] 张莹[1] 彭茵[1] 

机构地区：[1]大连医科大学检验学院

出　　处：《光谱实验室》2011年第1期165-168,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：采用水提醇沉法制备黄芪多糖提取物,苯酚-硫酸分光光度法测定多糖的含量,在0.00—0.14mg/mL线性范围内具有良好的线性关系,回收率在94.81%—103.50%。测定黄芪多糖含量为4.3%。采用校准曲线法,用空气-乙炔火焰原子吸收光谱法测定样品中Fe、Zn、Cu和Mn的含量,膜荚黄芪中微量元素含量较高。The content of polysaccharides in astragalus membranaceus（fisch） was determined by phenol-sulfuric acid spectrophotometry with extracting of water and purifying of EtOH.The method had a good recovery of 94.81%—103.50% in range of 0.00—0.14mg/mL.The content of polysaccharides was 4.3%.The contents of trace elements,Fe,Zn,Cu and Mn were determined by air-acetylene flame atomic absorption spectrometry.There were the higher contents of these elements in astragalus membranaceus of HeiLongjiang.

关 键 词：黄芪 多糖 微量元素 苯酚-硫酸分光光度法 原子吸收光谱法 

分 类 号：O657.31[理学—分析化学] O657.32[理学—化学]