手性固定相NP-HPLC拆分达非那新中间体对映体  

Separation of Darifenacin Intermediate Enantiomers by Normal-Phase HPLC with Chiral Stational Phase

作　　者：邹卫[1] 赵华[1] 赵静[1] 袁佩[2] 靳红卫[1] 

机构地区：[1]重庆医科大学药学院药物分析教研室,重庆市渝中区医学院路1号400016 [2]重庆医科大学生命科学院,重庆市400016

出　　处：《光谱实验室》2011年第1期188-191,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：采用多糖衍生物手性色谱柱,建立了正相高效液相色谱法(NP-HPLC)拆分达非那新中间体对映体。考察了该对映体在不同手性固定相上的分离趋势及保留顺序,以及流动相组成对该中间体对映体的分离度和洗脱顺序的影响。结果表明:用Chiralpak AD和Chiralcel OJ柱均能实现对映体的有效分离,为达非那新中间体的光学纯度检测提供了可靠的分离方法。A NP-HPLC method was established for the separation of darifenacin intermediate enantiomers with polysaccharide chiral column.Enantiomeric separation trend and retention order on different chiral stationary phases were investigated,as well as the separation and elution order by influence of mobile phase composition.The effective separation of enantiomers was obtained with Chiralpak AD and Chiralcel OJ column,which provided a reliable separation method for the optical purity determination of darifenacin intermediate.

关 键 词：达非那新中间体 手性固定相 对映体 分离 

分 类 号：O657.72[理学—分析化学]