吸入一氧化氮对急性大面积肺血栓栓塞家兔血浆vWF、GPⅡb/Ⅲa和CTnI的影响  

The effect of inhalation nitric oxide on CTnI vWF and GPⅡb/Ⅲaof rabbits with acute massive pulmonary embolism

作　　者：张泽明[1] 陈宝元[1] 葛常青[2] 张敬国[3] 王彦存[3] 赵学琴[3] 

机构地区：[1]天津医科大学总医院呼吸科,天津300402 [2]保定市第二医院 [3]河北大学附属医院呼吸科

出　　处：《中国实验诊断学》2011年第1期41-44,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的通过研究大面积肺血栓栓塞症家兔心肌受损模型的心肌肌钙蛋白(CTnI)浓,血浆血管性假血友病因子(vWF)和血小板膜表面糖蛋白(GPⅡb/Ⅲa)的改变及吸入一氧化氮对其影响,来探讨吸入一氧化氮对大面积肺血栓栓塞症家兔受损心肌保护作用的机理。方法日本大白兔30只,分为试验组10只,只对家兔进行大面积肺血栓栓塞症心肌受损模型实验;对照组10只:在实验组静脉注射血栓栓子时,本组动物模型制作只静脉注射生理盐水,不注射血栓栓子;治疗组10只:大面积肺血栓栓塞症动物模型成功后吸入一氧化氮治疗24小时,一氧化氮吸入浓度为40×10-6g/l。试验组、对照组、治疗组于试验组模型制备成功后每2 h,吸入一氧化氮治疗24 h同步抽血各一次,血浆心肌肌钙蛋白、血浆血管性假血友病因子、血小板膜表面糖蛋白被测定分别微粒子化学发光法流式细胞仪检测酶联免疫法。治疗组吸入一氧化氮治疗同时,试验组、对照组同步吸入50%氧气,多道生理参数分析记录仪,同步检测动脉平均压和肺动脉压。结果家兔大面积肺血栓栓塞症心肌受损动物模型制备成功后24小时后心肌肌钙蛋白浓度明显高于对照组(0.41±0.07,0.08±0.009,P<0.05),吸入一氧化氮24小时后治疗组明显低于实验组(0.31±0.07,0.41±0.07,P<0.05);急性大面积肺血栓栓塞症家兔栓塞后2小时和24小时其血浆vWF水平明显高于对照组(153.8±26.2,212.7±28.4P<0.05;111.2±24.6,113.1±25.1,P<0.05),吸入一氧化氮24小时后其浓度水平明显下降与实验组比较(131.8±25.4,212.7±28.4,P<0.05);家兔大面积肺血栓栓塞症栓塞后2小时和24小时其血浆GPⅡb/Ⅲa水平明显高于对照组(14.04±6.01,23.03±6.70,P<0.05;2.14±0.82,2.13±0.79,P<0.05),吸入一氧化氮24小时后其浓度水平明显下降与实验组比较(10.4±7.1,23.03±6.70,P<0.05);实验组模型制备成功后24小时血浆心肌肌钙蛋白与血浆vWF、GPⅡbObjective By studying the change of on cardiac troponin I,Von Willebrand factor（vWF）,GPⅡb/Ⅲ of rabbits with acute massive pulmonary embolism and these be affacted by inhalation nitric oxide,investigate the reason that is damage cardiac muscle of rabbits with acute massive pulmonary embolism is protected by inhalation nitric oxide.Methods 30 Japanese white rabbits were randomly divided into the experimental group（cute massive pulmonary embolism）,contrast group,contral group（onely inject physyologicall salt into the vein）,treatment group（cute massive pulmonary embolism rabbits inhalated nitrin oxide of 40×10-6g/l）.The concentrationand of cardiac troponin I,vWF,GPⅡb/Ⅲ was measured at 2 h for 24 h after animals models has successfully been finished.Results the cardiac troponin I of rabbits with acute massive pulmonary embolism were significantly higher than that control group at 24 h after animals models has successfully been finished（0.41±0.07,0.08±0.009,P0.05）,the cardiac troponin I in treatment group were significantly lower than that experimental group after 24 h animals inhalated nitrin oxide（0.31±0.07,0.41±0.07,P0.05）;the level of vWF of rabbits with acute massive pulmonary embolism were significantly higher than that control group after 2 h and 24 h in the animals models has successfully been finished（153.8±26.2,212.7±28.4 P0.05;111.2±24.6,113.1±25.1,P0.05）,the level of vWF in treatment group were significantly lower than that experimental group after 24 h animals inhalated nitrin oxide（131.8±25.4,212.7±28.4,P0.05）,the level of GPⅡb/Ⅲ of rabbits with acute massive pulmonary embolism were significantly higher than that control group after 2 h and 24 h in the animals models has successfully been finished（14.04±6.01,23.03±6.70,P0.05;2.14±0.82,2.13±0.79,P0.05）,the level of GPⅡb/Ⅲ in treatment group were significantly lower than that experimental group after 24 h animals inhalated nitrin oxide（10.4±7.1,23.03±6.70,P0.05）the relative was markedl

关 键 词：肺血栓栓塞症 家兔 吸入一氧化氮 血浆血管性假血友病因子 血小板膜表面糖蛋白 

分 类 号：R563.5[医药卫生—呼吸系统]