CpG ODN诱导人PBMC对结肠癌细胞体外杀伤作用的研究  

Antitumor effect of human PBMC on colon carcinoma cells induced by CpG ODN

作　　者：韩刚[1] 李金龙[1] 贾明库[1] 

机构地区：[1]吉林大学第二医院普通外科,吉林长春130041

出　　处：《中国实验诊断学》2011年第1期89-90,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅资助项目(项目编号20050410-5)

摘　　要：目的以CpG ODN为免疫佐剂激活人外周血单个核细胞(PBMC),观察激活的PBMC对肿瘤细胞的杀伤活性。方法分别以肿瘤抗原(Ag)、白细胞介素-2(IL-2)和CpG ODN单独或联合刺激正常人和肿瘤患者PBMC,测定其对肿瘤细胞的杀伤活性。结果以CpG ODN刺激人PBMC组的细胞,其对肿瘤细胞的杀伤活性明显高于对照组(P<0.05)。结论 CpG ODNI、L-2与肿瘤抗原共同刺激能有效提高人PBMC的杀伤活性。Objective Activate PBMCs with CpG ODN which acted as immunoadjuvant and observe their killing activity to the cancer cells.Methods Stimulate PBMCs of health adults and patients suffered from cancer with tumor antigen,IL-2 or CpG ODN and determine their killing activity to the cancer cells.Results The cell proliferation activity value of CpG ODN stimulating group is higher than the other groups（P0.05）.Conclusion The activity of human PBMC can be improved effectively with the stimulations of CpG ODN,IL-2 and tumor antigen together.

关 键 词：CPGODN 结肠癌 人外周血单核细胞 

分 类 号：R735.35[医药卫生—肿瘤]