部分鸢尾属植物的AFLP标记  

Genetic Diversity of Iris Determined by AFLP Markers

作　　者：牟少华[1] 彭镇华[2] 郄光发[2] 孙振元[2] 

机构地区：[1]国际竹藤网络中心,北京100102 [2]中国林业科学研究院林业研究所

出　　处：《东北林业大学学报》2011年第1期124-126,共3页Journal of Northeast Forestry University

基　　金：国家科技基础条件平台项目(2005DKA21003)

摘　　要：利用AFLP技术，选择EcoR／／MesI酶切组合，从48对EcoRI＋3／iVIesI＋3引物组合中筛选出6对引物进行选择性扩增，检测了鸢尾属植物26个样品基因组的DNA多态性，共扩增出536个遗传位点，并通过Jaccard的方法将电泳谱带矩阵转化为遗传相似性系数矩阵，进行了UPGMA聚类分析。26个样品的遗传相似性系数（GS）为0．56～0．89，表现出丰富的遗传多样性。阈值取0．624时，可以将所有样品分为6组：第1组为鸢尾和其变种白花鸢尾；第2组为马蔺；第3组为野鸢尾和小鸢尾；第4组为长白鸢尾；第5组为粗根鸢尾；第6组为其余18个样品。大多数同种鸢尾的不同种源首先聚在一起，相似性系数较高，亲缘关系也较近。The Amplified Fragment Length Polymorphism （AFLP） technique was used to analyze genetic diversity of 26 samples of lr/s collected from China. Genomic DNA was digested with EcoRI and MseI enzymes and amplified with 6 EcoRI＋3/MesI＋3 primer combinations. A total of 536 scorable bands were produced by AFLP analysis, which were translated into genetic similarity matrix. Genetic similarity, ranging from 0.56 to 0.89, showed abundant genetic diversity. Cluster analysis was made based on the UPGMA method. All the samples were classified into six groups. The first group consisted of I. tectorum and I. tectorum f. alba, the second one of I. lactea, the third one of I. dichotoma and I. proantha, the fourth one of I. mandshurica, the fifth one of I. tigridia, and others were clustered into the sixth group. Different provenances of Iris belonging to the same species with high genetic similarity were closely correlated and gathered together.

关 键 词：鸢尾属 AFLP 多样性 亲缘关系 

分 类 号：Q943.2[生物学—植物学] Q949.718.28