假蕈状芽孢杆菌纤溶酶基因的克隆与表达  

Cloning and Expression of Fibrinolytic Enzyme Gene from Bacillus pseudomycoides

作　　者：郭晓军[1] 袁洪水[1] 刘慧娟[1] 张冬冬[1] 朱宝成[1] 

机构地区：[1]河北农业大学生命学院,河北保定071001

出　　处：《中国医药工业杂志》2011年第1期14-16,65,共4页Chinese Journal of Pharmaceuticals

摘　　要：将来自假蕈状芽孢杆菌的纤溶酶基因克隆至表达载体pGEX-4T-2中，并在大肠杆菌中表达。SDS—PAGE结果显示胞内含有表达条带，表达产物的相对分子量大小为9．8×10^4，比预计的分子量（90×10^4）稍大，酪蛋白平板法测定结果显示，以1．0mmol／L IPTG26℃和30℃诱导5h，重组细胞内蛋白有活性。The fibrinolytic enzyme （BpFE） gene originated from Bacillus pseudomycoides was inserted into the expression vector pGEX-4T-2 and then expressed in Escherichia coli. SDS-PAGE analysis was performed after IPTG induction at low temperature. The apparent molecular weight of the expression product was 9.8×10^4, slightly larger than the prediction （9.0×10^4）. The results of casein-plate method showed that the intracellular protein from recombinant when induced with 1.0 mmol/L IPTG for 5 h at 26 ℃ and 30 ℃ had biological fibrinolytic activity.

关 键 词：纤溶酶 假蕈状芽孢杆菌 克隆 表达 

分 类 号：Q78[生物学—分子生物学]