铜和维生素A及其互作效应对肉鸡免疫功能的影响  

Influence of dietary copper and vitamin A on immune function of broilers

作　　者：古少鹏[1] 郑明学[1] 李宝钧[1] 张春善[2] 申红星[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]河北科技师范学院动物科学系,昌黎066600

出　　处：《中国生态农业学报》2011年第1期135-140,共6页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金项目(30771619);山西省自然科学基金项目(20031072)资助

摘　　要：采用4×2（铜×维生素A）完全随机试验设计,研究了日粮添加不同水平的铜（0mg·kg-1、8mg·kg-1、150mg·kg-1、225mg·kg-1）和维生素A（1500IU·kg-1、5000IU·kg-1）对肉仔鸡不同生长阶段（0~4周龄和5~7周龄）免疫功能的影响。结果表明：添加铜为8mg·kg-1时,肉鸡生长前期（0~4周龄）可显著（P〈0.05）或极显著（P〈0.01）提高肉鸡脾脏活化T淋巴细胞百分率和血清抗体效价,生长后期（5~7周龄）可极显著（P〈0.01）或显著（P〈0.05）提高脾脏、胸腺、盲肠扁桃体活化T淋巴细胞百分率和外周血液中活化T、B淋巴细胞百分率;整个试验期添加高铜（150mg·kg-1、225mg·kg-1）时,活化T、B淋巴细胞百分率均不同程度下降。添加维生素A5000IU·kg-1时,肉鸡生长前期可显著（P〈0.05）提高其外周血液中活化T淋巴细胞百分率,生长后期可极显著（P〈0.01）或显著（P〈0.05）提高脾脏、盲肠扁桃体活化T淋巴细胞百分率和血清抗体效价。铜和维生素A互作效应对肉鸡生长前期的活化T、B淋巴细胞百分率和血清抗体效价影响不显著,对生长后期的外周血液、胸腺、盲肠扁桃体活化T淋巴细胞百分率影响显著（P〈0.05）,以8mg·kg-1铜×5000IU·kg-1维生素A处理最高;以上结果提示铜和维生素A对肉鸡免疫功能的影响存在互作效应。A 4×2 （Cu×VA） random experiment was designed to investigate the effect of different dietary levels of Cu （0 mg·kg-1, 8 mg·kg-1, 150 mg·kg-1, 225 mg·kg-1） and VA （1 500 IU·kg-1, 5 000 IU·kg-1） on broiler immune system at 0~4 and 5~7 week-old. The results showed significant effects （P0.05, P0.01） of Cu at 8 mg·kg-1 on percentage of activated T-lymphocytes in spleen and serum antibody titers of 0~4 week-old broilers. At the same Cu level, percentage of activated T-lymphocytes in spleen, thymus and cecal tonsil, and percentage of activated B/T-lymphocytes in peripheral blood significantly increased （P0.05, P0.01） in 5~7 week-old broilers. Percentage of activated B/T-lymphocytes decreased at different degrees under high Cu levels of 150~225 mg·kg-1 feed. At VA supplemental level of 5 000 IU·kg-1 feed, percentage of activated T-lymphocytes in peripheral blood also significantly increased （P0.05） for the 0~4 week-old broilers, while percentage of activated T-lymphocytes in spleen, cecal tonsil and serum antibody titers significantly increased （P0.05, P0.01） for the 5~7 week-ld broilers. Percentage of activated B/T-lymphocytes and serum antibody titers were not significantly affected by Cu/VA interaction for 0~4 week-old broilers. However, percentage of activated T-lymphocytes of cecal tonsil, thymus and peripheral blood significantly increased （P0.05） for 5~7 week-old broilers, especially under Cu （8 mg·kg-1） × VA （5 000 IU·kg-1） interaction. Cu/VA interaction had a significant effect on the function of broiler immune system.

关 键 词：肉仔鸡 铜 维生素A 活化淋巴细胞 血清抗体 互作效应 

分 类 号：Q562[生物学—生物化学] S816.72[农业科学—饲料科学]