老年患者血清梅毒抗体酶联免疫吸附试验假阳性结果分析  

Analysis of false positive results by ELISA analysis in elderly syphilis

作　　者：邓红艳[1] 赫兰辉[1] 

机构地区：[1]成都大学附属医院检验科,成都610081

出　　处：《检验医学与临床》2011年第2期151-151,153,共2页Laboratory Medicine and Clinic

摘　　要：目的对老年患者血清梅毒抗体假阳性结果及与临床的相关性进行分析和探讨。方法采用梅毒螺旋体-酶联免疫吸附试验(TP-ELISA)分别检测2 454例60岁以上老年患者和12 545例60岁以下患者血清中梅毒特异性抗体,阳性结果再用梅毒螺旋体明胶凝集试验(TPPA)复查。结果 60岁以上老年患者TP-ELISA假阳性率明显高于60岁以下患者,两组间差异有统计学意义(P<0.05)。结论试剂本身、人类宿主相关的螺旋体、基础疾病等因素是老年患者TP-ELISA易发生假阳性的重要原因,应防止产生误诊导致的医疗纠纷。Objective To analyze the false positive results of syphilis antibodies detected by ELISA in elderly patients with syphilis and to study their relation with clinic.Methods TP-ELISA was used to detect anti-syphilis specific antibody in 2454 cases of syphilis aged more than 60 years and 12,545 cases of syphilis aged less than 60 years in our hospital from January 2007 to January 2010,those with positive results were redetected by TPPA.Results The false-positive rate of ELISA-TP in the patients aged over 60 was significantly higher than that in the patients aged less than 60 with statistical difference between the two groups（P0.05）.Conclusion The reagent itself,human hosts-related spirochetes,basic diseases are the the major reasons easily causing the false positive results of TP-ELSA in elderly syphilis.Should prevent misdiagnosis to result in medical disputes.

关 键 词：梅毒 抗体 细菌 酶联免疫吸附测定 假阳性反应 老年人 

分 类 号：R512.62[医药卫生—内科学]