拟南芥低盐敏感突变体的筛选  

Identifying Low Concentration of Salt-sensitive Mutants of Arabidopsis

作　　者：张丽霞[1,2] 李卓夫[2] 杨德鑫[2] 王瑞刚[3] 李国婧[1,3] 黄荣峰[2] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]中国农业科学院生物技术研究所,北京100081 [3]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《生物技术通报》2011年第1期90-94,共5页Biotechnology Bulletin

基　　金：国家基础研究项目(2006CB100102);国家自然科学基金项目(90917018)

摘　　要：通过对ein3-1功能缺失型突变体种子进行EMS诱变,筛选到47株盐敏感突变体。根据对盐敏感程度的不同将其分为3类,分别为低盐超敏感突变体(low concentration of salt hyper-sensitive mutants,lsh),低盐中等敏感突变体(low con-centration of salt moderate-sensitive mutants,lsm)和低盐弱敏感突变体(lowconcentration of salt slight-sensitive mutants,lss)。以其中一株lss-3为例,进行了深入研究。根据遗传分析和生理试验表明,lss-3是以ein3-1为背景的隐性双突变体,而且具有比Col-0和ein3-1更加敏感的盐表型。三重反应表明,lss-3与ein3-1类似,表现出对ACC不敏感的表型。推测lss-3突变的基因可能与乙烯信号途径组分EIN3有关,也可能与之无关,仅是参与抗盐的一个新基因。In order to screen and identify low concentration of salt-sensitive mutants,we generated an Arabidopsis ein3-1 mutant pool by ethyl methanesulfonate（EMS）.Based on the fact that Arabidopsis ein3-1 seedlings are sensitive to 100 mmol/L NaCl,we then screened and identified ca.47 putative sensitive mutants from the ein3-1 seed-mutagenized mutants with 75 mmol/L NaCl.These mutants can be classified into low concentration of salt hyper-sensitive mutants（lsh）,low concentration of salt moderate-sensitive mutants（lsm） and low concentration of salt slight-sensitive mutants（lss）.Furthermore,we take an example of lss-3 mutant to study in depth.Genetic analysis indicates that lss-3 is a recessive mutant,which is more sensitive to 75 mmol/L NaCl than Col-0 and ein3-1,indicating that lss-3 gene might be associated with salt response.Physiological test suggests that the mutation of lss-3 keeps ＂the triple response＂ of ein3-1 single mutant,indicating that the alteration of salt sensitivity in lss-3 mutant is possibly associated with the ethylene signaling component of EIN3,or a new gene is in connection with salt stress.

关 键 词：ein3-1 甲基磺酸乙酯 低盐敏感突变体 乙烯信号转导途径 

分 类 号：Q943[生物学—植物学]