绵羊DRB1基因生物信息学分析  

Bioinformatics Analysis of Sheep DRB1 Gene

作　　者：徐飞[1] 成述儒[1] 罗玉柱[1] 

机构地区：[1]甘肃农业大学动物科学技术学院甘肃省草食动物生物技术重点实验室,兰州730070

出　　处：《生物技术通报》2011年第1期113-118,共6页Biotechnology Bulletin

基　　金：国家高技术研究发展计划项目("863"计划)(2006AA10Z196);国家科技支撑计划项目(2007BAD52B05);甘肃省农业生物技术专项(GNSW-2009-24);甘肃省重点实验室建设计划项目(085RTSA004)

摘　　要：利用生物基因组学数据库,对绵羊MHCⅡ区DRB1基因进行生物信息学分析,以预测DRB1基因编码产物的理化性质、结构与功能,同时构建DRB1同源基因的系统进化树。结果表明,DRB1基因编码产物为不稳定亲水性蛋白,具有明显的信号肽,其切割位点位于29-30位的氨基酸之间。二级结构主要以无规则卷曲和延伸链为主,主要在细胞质中发挥生物学作用。序列分析表明,DRB1编码产物可能具有免疫应答和受体、胁迫应答和信号转导等功能,可能在免疫应答和胁迫应答过程中发挥重要作用,可能对绵羊免疫力和抗病性起关键作用。DRB1基因编码产物氨基酸邻接系统树表明,绵羊DRB1与山羊、羚羊、塔尔羊和牛等物种DRB1氨基酸距离较近,具有高度同源性。This study aimed to bioinformatics of DRB1 in ovine MHCⅡarea.The physicochemical characteristics,structures and functions of ovine MHC-DRB1 were predicted and analyzed with software tools and database.Meanwhile,the phylogenetic tree of DRB1 and related proteins was constructed.The result showed that OLA-DRB1 is unstable hydrophilic proteins,which has obvious signal peptide.The secondary structure was primarily composed of random coil and sheet,and performs biological effects in the cytoplasm.Sequence analysis indicated that this gene might involve in immune response,receptor,stress response and signal transducer roles,and play important roles in immune response and stress response.The results suggested that MHC-DRB1 might play a key role in immunity and disease resistance in ovine.The DRB1 and related proteins of Ovine,Capra hircus,Rupicapra rupicapra,Hemitragus jemlahicus,Bos taurus are close in the phylogenetic tree,which showed highly homology.

关 键 词：主要组织相容性复合体 DRB1基因 生物信息学 绵羊 

分 类 号：S826[农业科学—畜牧学] Q78[农业科学—畜牧兽医]