间接ELISA检测抗传染性法氏囊病病毒抗体方法的建立  

Development of an Indirect ELISA to Detect Antibody Agaist Infectious Bursal Disease Virus

作　　者：宋军[1] 黄成斌[1] 潘玲[1] 

机构地区：[1]安徽农业大.学动物科技学院,安徽合肥230036

出　　处：《中国家禽》2011年第1期15-17,共3页China Poultry

摘　　要：采用原核表达的传染性法氏囊病毒(IBDV)核衣壳蛋白VP2作为诊断抗原,建立检测IBD血清抗体的间接ELISA方法。抗原最佳包被量为每孔174.67ng,待检血清最佳稀释度为1∶40,待检血清样品的D492nm≥0.43Dpos+0.57Dneg判为阳性,反之判为阴性。对采自安徽省部分地区的979鸡血清样品进行检测,IBDV抗体阳性率为39.73%。结果证实,间接ELISA法用于IBDV血清抗体的监测具有良好的特异性,是一种快速简便的血清学方法,值得在基层推广应用。Using recombinant nucleocapsid VP2 of infectious bursal disease virus （IBDV） as antigen, an indirect ELISA for the detection of antibodies against IBDV was developed. The optimal coating concentration of antigen was amounted to 174.67ng of VP2 protein per well,the serum sample for testing was diluted to 1：40 and the cutoff was determined to be 0.43 Dpos＋0.57 Dneg. A total of 979 serum samples originated from different areas in Anhui province were detected by this indirect ELISA,and the positive rate was 39.73%. The results confirmed that indirect ELISA was specific to monitor serum antibody of IBDV. This detection method was quick and easy,and it is deserved to promote and use in practice.

关 键 词：传染性法氏囊病病毒 VP2蛋白 间接ELISA 抗体检测 

分 类 号：S852.659.4[农业科学—基础兽医学]