DNA/羟基磷灰石杂化膜修饰电极用于研究DNA与量子点的相互作用  

Study on the interaction of DNA with quantum dots using DNA/hydroxyapatite composite film modified electrode

作　　者：刘燕[1] 李静[1] 马荣娜[1] 赵倩[1] 王国涛[1] 贾文丽[1] 王怀生[1] 

机构地区：[1]聊城大学化学化工学院分析化学研究所,聊城252059

出　　处：《中国科学：化学》2011年第1期30-36,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(20475024&20775031);山东省"泰山学者"建设工程专项经费资助

摘　　要：首次利用溶胶-凝胶一步法制备了纳米多孔羟基磷灰石(HAp)-DNA杂化膜修饰玻碳电极,HAp优良的生物相容性和独特的吸附性可以将DNA固定在HAp多孔薄膜上,而DNA的大分子结构对HAp膜起到稳定剂的作用.采用循环伏安法和交流阻抗法系统地研究了电极表面固定DNA的稳定性以及固定的DNA与非电活性核壳型量子点CdTe/CdS之间的相互作用.实验结果表明,DNA可以牢固地固定在HAp多孔膜上并满足电化学研究的需要,量子点与DNA之间作用方式主要为插入作用,这种插入作用可以导致DNA的内部结构变化,与圆二色谱法得到的结果一致,并计算出了DNA与量子点的作用常数及吉布斯自由能变化.该研究结果对进一步研究量子点的毒性具有重要的参考价值,同时也为利用电化学方法研究DNA与非电活性物质的相互作用提出一个新的思路.Nano-porous hydroxyapatite （HAp）-DNA composite film modified glassy carbon electrode was prepared by sol-gel one-step method for the first time. DNA was immobilized on the nano-porous HAp film firmly due to its effective adsorbability and biocompatibility and the structure of macromolecule of DNA plays an important role in the stabilization of HAp film. Studies on the interaction of DNA with non-electroactive core-shell CdTe/CdS quantum dots were carried out using the DNA/HAp modified electrode by cyclic voltammetry and impedance analysis. The results showed that DNA immobilized on the HAp film was stably enough for the electrochemical studies and there exists a remarkable intercalative interaction mode between QDs and DNA and the intercalative interaction could influence the configuration of DNA. This studies can provide important theoretical basis for the study on the toxicity of QDs and a new thinking for the study on the interaction of DNA with non-electroactive molecules using electrochemical methold.

关 键 词：DNA 羟基磷灰石 CDTE/CDS量子点 杂化膜修饰电极 圆二色谱法 

分 类 号：Q523[生物学—生物化学]