基于碳酸钙-金纳米粒子无机杂化复合物固定硫堇的新方法及其在过氧化氢传感器中的应用  

A novel strategy for immobilization of thionine based on calcium carbonate-gold nanoparticles inorganic hybrid composite and its application in hydrogen peroxide sensor

作　　者：李峰[1] 冯艳[1] 李晶晶[1] 郭杰[2] 

机构地区：[1]生态化工教育部重点实验室青岛科技大学化学与分子工程学院,青岛266042 [2]吉林大学第二医院放疗科,长春130025

出　　处：《中国科学：化学》2011年第1期66-73,共8页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金资助项目(20775039)

摘　　要：通过静电作用,经碳酸钙-金纳米粒子(CaCO3-AuNPs)无机杂化复合物实现了电活性物质硫堇(Th)在金电极表面的有效固定.AuNPs静电吸附到CaCO3微球表面形成CaCO3-AuNPs无机杂化复合物,该杂化复合物具有微孔结构、大的表面积和好的生物相容性,使得Th的固定量和稳定性大大提高.探讨了Th修饰电极在过氧化氢(H2O2)传感器中的应用,该传感器对H2O2的响应快速,线性范围宽(8.0×10-7～1.06×10-3mol/L),检测限为2.0×10-7mol/L.A novel strategy for efficient immobilization of electroactive Thionine （Th） on gold （Au） electrode surface based on calcium carbonate-gold nanoparticles （CaCO3-AuNPs） inorganic hybrid composite was proposed. It was conducted by the strong electrostatic interaction between positively charged Th and negatively charged CaCO3-AuNPs composite. The hybrid composite was obtained by the adsorption of AuNPs onto the surface of CaCO3 microspheres through electrostatic interaction. Due to the microporous architecture, large surface area, and good biocompatibility of CaCO3-AuNPs composite, the amount and stability of the immobilized Th were highly strengthened. The application of the resulting Th modified electrode in hydrogen peroxide （H2O2） sensor was also investigated and it exhibited rapid response to H202 within 3 s. The linear calibration ranged from 8.00 ×10^-7to 1.06 × 10^3 mol/L with a detection limit of 2.00 ×10^-7 mol/L.

关 键 词：硫堇 CaCO3-AuNPs 传感器 静电作用 

分 类 号：Q819[生物学—生物工程]