羊栖菜对实验性高脂血症大鼠血脂水平的调节作用  

Regulating effect of Hizikia fusiformis on serum lipid levels of hyperlipidemia rats

作　　者：于竹芹[1,2] 曾锋 徐新颖[1] 帅莉[4] 李生尧 段德麟[7] 

机构地区：[1]青岛大学医学院脑血管病研究所 [2]青岛大学医学院松山医院,山东青岛266021 [3]青岛国风药业股份有限公司,山东青岛266071 [4]青岛大学化学化工与环境学院,山东青岛266021 [5]浙江洞头县鹿丰羊栖菜研究所 [6]洞头县水产科学技术研究所,浙江温州325700 [7]中国科学院海洋研究所,山东青岛266071

出　　处：《中国海洋药物》2011年第1期45-49,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(40976085);山东省农业良种工程项目(N2006Y11B)

摘　　要：目的探讨羊栖菜对高脂血症大鼠血脂的调节作用。方法健康雌性Wistar大鼠40只,高脂饲料喂养建立高脂血症动物模型,羊栖菜粉饲料喂养干预治疗。生化法检测大鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平及脂蛋白酯酶(LPL)和肝脂酶(HL)活性。酶联免疫吸附法测定羟甲基戊二酰辅酶A(HMG-CoA)水平和羟甲基戊二酰辅酶A还原酶(HMG-CR)活性。结果羊栖菜治疗组动物血清TG、TC和LDL水平较模型组显著降低(P<0.05),而HDL水平显著升高(P<0.05);血清和肝组织中LPL和HL水平均显著高于模型组(P<0.05),肝组织HMG-CoA水平显著高于模型组、而HMG-CR的活性显著低于模型组(P<0.05)。结论羊栖菜可能通过增强LPL、HL和HMG-CR的活性,影响血脂组分的代谢,发挥调节血脂水平的作用。Objective To investigate the regulating effects of Hizikia fusiformis on serum lipid levels in experimental hyperlipidemia rats.Methods Forty healthy female Wistar rats were used to establish hyperlipemia models by feeding fat-rich forage,and the Hizikia fusiformis was applied as raw materials added into the forage.The levels of serum lipid including triglyceride（TG）,total cholesterol（TC）,low-density lipoprotein（LDL）,high-density lipoprotein（HDL） and the activities of lipoproteinesterase（LPL） and hepatic lipase（HL） were detected by biochemical assay.Enzyme-linked immunosorbent assay（ELISA） was applied to determine the level of 3-hydroxy-3-methylglutaryl coenzyme A（HMG-CoA） and activity of HMG-CoA reducase（HMG-CR）.Results After treated with Hizikia fusiformis,the levels of serum TG,TC and LDL decreased while the serum HDL increased significantly than those of model group（P0.05）.The levels of LPL and HL in serum and hepatic tissue were significantly higher than those in model group（P0.05）,and the HMG-CoA level of hepatic tissue was significantly higher while the HMG-CR activity lower than those of model group（P0.05）.Conclusion Hizikia fusiformis might play a regulating effect on serum lipid by enhancing the activities of LPL and HL and inhibiting the activity of HMG-CR to interfere the metabolism of TG,TC,LDL and HDL.

关 键 词：羊栖菜 高脂血症 脂蛋白酯酶 肝脂酶 羟甲基戊二酰辅酶A还原酶 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]