抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用  

Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker-Assisted Selection Breeding

作　　者：殷得所[1] 夏明元[1] 李进波[1] 万丙良[1] 査中萍[1] 杜雪树[1] 戚华雄[1] 

机构地区：[1]湖北省农业科学院粮食作物研究所,湖北武汉430064

出　　处：《中国水稻科学》2011年第1期25-30,共6页Chinese Journal of Rice Science

基　　金：湖北省攻关重大专项资助项目(2006AA201A03);国家863计划资助项目(2006AA100101);国家科技支撑计划资助项目(2006BAD01A01)

摘　　要：利用带有广谱抗稻瘟病基因Pi9的籼稻品系75-1-127作为抗病基因供体亲本,用于扬稻6号和R6547抗病性的回交育种。通过比较75-1-127、扬稻6号和日本晴的Pi9基因位点的DNA序列,开发出了与Pi9基因紧密连锁的共显性STS(序列标记位点)标记PB9-1,用于Pi9基因的分子标记辅助选择。结合田间农艺性状选择和分子标记辅助选择,培育出8个Pi9基因纯合的回交后代株系。其中,具有扬稻6号和R6547遗传背景的株系各4个。经湖北恩施和宜昌的病圃鉴定,携带有抗病基因株系的稻瘟病抗性水平较受体品种扬稻6号和R6547有不同程度的提高。具有R6547遗传背景的株系08C893配制的杂交组合在上述病区的抗性表现也明显优于对照品种扬两优6号。上述结果说明,共显性标记PB9-1在Pi9抗稻瘟病基因分子标记辅助育种中具有应用价值,并且Pi9基因作为稻瘟病抗源之一可以在湖北稻区进行有效利用。Indica rice line 75-1-127 carrying broad-spectrum rice blast resistance gene Pi9 was used as resistance gene donor for genetic improvement of Yangdao 6 and R6547.By alignment of genomic sequences from the Pi9 locus of 75-1-127,Yangdao 6 and Nipponbare,co-dominant STS（sequence-tagged site） marker PB9-1,which linked to the Pi9 gene,was developed for molecular marker-assisted selection（MAS）.Eight backcross lines carrying Pi9 were obtained,among which four lines contained the genetic background of Yangdao 6 and the other four lines had the R6547 background.Under natural disease infection conditions in Enshi County and Yichang County of Hubei Province,China,the Pi9-carrying lines showed higher blast disease resistance than Yangdao 6 and R6547 in various degrees.The hybrids originated from 08C893,an R6547-derived rice line,showed higher blast disease resistance as compared to the control Yangliangyou 6.The results suggest PB9-1 is an effective co-dominant marker for MAS of Pi9 which is a valuable resistance gene for the control of rice blast disease in Hubei area.

关 键 词：稻瘟病抗性 分子标记辅助选择 序列标记位点 抗性基因 育种 水稻 

分 类 号：S511[农业科学—作物学]