棉花芽黄突变体十个叶绿体蛋白编码基因RNA编辑位点的测定及分析  

The Identification and Analysis of RNA Editing Sites of 10 Chloroplast Protein-coding Genes from Virescent Mutant of Gossypium hirsutum

作　　者：江媛[1] 何筠[1] 范术丽[2] 俞嘉宁[1] 宋美珍[2] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062 [2]中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南安阳455000

出　　处：《棉花学报》2011年第1期3-9,共7页Cotton Science

基　　金：转基因生物新品种培育重大专项(2008ZX08005-002)

摘　　要：RNA编辑是陆生植物叶绿体转录后基因表达调控的一种方式。已有研究表明植物黄化或白化可能与叶绿体RNA编辑有关。本实验采用PCR,RT-PCR及测序的方法,确定棉花芽黄突变体V1子叶与真叶的10个叶绿体蛋白编码基因中各有34个编辑位点,有6个位点存在编辑效率的差异。将这些编辑位点与柯字310比较发现,芽黄突变体多5个编辑位点。运用生物信息学软件对34个编辑位点进行分析,结果表明accD-109,clpP-187,ndhB-50,ndhB-196,ndhD-128,ndhD-225等13个位点会影响相应蛋白二级或三级结构,表明上述编辑位点的改变可能对其蛋白的正确组装及功能的发挥起重要作用。RNA editing is one of the post-transcriptional regulation mechanisms of gene expression in the chloroplast of land plants.Recently,studies have shown that albino or yellow phenotype of high plants may be relevant to chloroplast RNA editing.In this paper,we investigated the RNA editing sites of 10 plasmid protein-coding genes from cotyledons and leaves of virescent mutant V1,Gossypium hirsutum,using PCR,RT-PCR and sequencing methods.There were 34 editing sites in both of them,but the editing efficiency was different in 6 sites of 34.Compared all editing sites between V1 and Coker310FR,we found that V1 had 5 novelty editing sites in accD-109,accD-468,ndhD-347,rpoA-69,and rpoA-279.Analyzed all the editing sites by using bioinformatics,the results showed that 13 sites,accD-109,clpP-187,ndhB-50,ndhB-196,ndhD-128,ndhD-225 and so on,would affect their protein secondary structures or three-dimensional structures.All the results indicate that the above-mentioned sites may play important roles in proteins assemble correctly and execute their function effectively.

关 键 词：叶绿体 陆地棉 芽黄突变体 RNA编辑 

分 类 号：S562[农业科学—作物学]