电脉冲激活猪卵母细胞的研究  

Electro activation of the Porcine Oocytes

作　　者：陈乃清[1] 赵浩斌[2] 

机构地区：[1]武汉大学生命科学学院,武汉430072 [2]湖北省农业科学院畜牧兽医研究所,武汉430209

出　　处：《武汉大学学报（自然科学版）》1999年第4期463-465,共3页Journal of Wuhan University(Natural Science Edition)

基　　金：国家自然科学基金

摘　　要：通过对猪卵母细胞的电激活进行系统研究，结果表明：对于体外成熟卵母细胞，电场强度为 １ ２５０ Ｖ／ｃｍ ，脉冲时程为４０、６０ μｓ时，效果较好，一次脉冲激活率可达８５．３％ 和８７．１％ ；而对于体内成熟卵母细胞，电场强度为１ ５００ Ｖ／ｃｍ ，脉冲时程为４０、６０ μｓ 时，效果较好，一次脉冲激活率达８５．７％ 和８８．２％ ；增加脉冲次数并不能显著提高激活率，反而使卵母细胞裂解率显著增加；激活液选用０．３ ｍ ｏｌ／ Ｌ甘露醇（含０．１ ｍ ｍ ｏｌ／ Ｌ Ｍｇ Ｓ Ｏ４ ）时，添加 ０．１ ｍ ｍ ｏｌ／ Ｌ Ｃａ Ｃｌ２ ，获得较好的激活率（８７．８％ ）电脉冲激活卵母细胞后进一步培养。The techniques on electro activation of the porcine oocytes were systematically studied. In the study of electroactivation, parthenogenetical activation rate was high to 85.3%～87.1% when pulse were 40～60 μs, when direct current field strength was 1 250 V/cm for oocytes maturated in vitro; and 85.7%~88.2% when direct current field strength was 1 500 V/cm for oocytes matured in vivo. Multiple pulses could not increase significantly parthenogenetical activation rate, but increased oocytes lysis rate. When nonelectrolyto solution (0.3 mol/L Mannital +0.1 mmol/L MgSO 4+0.1 mmol/L CaCl 2) was used as electro activation medium, parthenogenetical activation rate was high to 87.8%.

关 键 词：卵母细胞 猪 电脉冲激活 核移植克隆动物 

分 类 号：S828.3[农业科学—畜牧学] S814[农业科学—畜牧兽医]