二氧化硫预处理对心肌缺血再灌注损伤大鼠二氧化硫/天门冬氨酸氨基转移酶体系的影响  

Effect of Sulfur Dioxide Preconditioning on Sulfur Dioxide/Aspartate Amino Transferase Pathway in Rats with Myocardial Ischemia-Reperfusion Injury

作　　者：王新宝[1] 金红芳[1] 唐朝枢[2,3] 杜军保[1,3] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学医学部生理与病理生理学系,北京100191 [3]教育部分子心血管学重点实验室,北京100191

出　　处：《实用儿科临床杂志》2011年第1期9-11,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(81070111);国家自然科学基金创新研究群体科学基金(30821001);国家长江学者奖励计划

摘　　要：目的探讨二氧化硫(SO2)预处理对心肌缺血再灌注损伤大鼠的保护作用及对心肌组织中SO2/天门冬氨酸氨基转移酶(AAT)体系的影响。方法 24只健康清洁级雄性Wistar大鼠随机分为假手术组、缺血再灌注组(I/R组)、SO2预处理组(I/R+S组)。结扎大鼠左侧冠状动脉30min,再灌注120min,制备心肌缺血再灌注模型;I/R+S组大鼠在缺血前10min用1μmoL.kg-1SO2颈外静脉注射预处理。采用全自动生化检测仪检测大鼠血浆CK和LDH水平,用高效液相色谱法测定其心肌组织中SO2水平,采用蛋白免疫印迹法检测其心肌组织AAT1和AAT2蛋白表达水平。结果缺血再灌注后,I/R组及IR+S组血浆CK、LDH水平均较假手术组明显增高(P<0.01,0.05),I/R+S组血浆CK、LDH水平较I/R组均明显降低(Pa<0.05)。与假手术组比较,I/R组大鼠心肌组织匀浆中SO2水平显著降低(P<0.05),I/R+S组无明显变化;I/R+S组SO2水平较I/R组显著增高(P<0.01)。I/R组大鼠心肌组织AAT1蛋白表达较假手术组显著降低(P<0.01),I/R+S组与假手术组比较差异无统计学意义(P>0.05),而I/R+S组AAT1蛋白表达则较I/R组显著增高(P<0.01);AAT2蛋白表达在3组间的差异无统计学意义(P>0.05)。结论 SO2预处理可明显降低心肌缺血再灌注大鼠血浆中心肌酶活性,具有心肌保护作用,并对心肌组织中SO/AAT体系具有上调作用。Objective To investigate the effect of sulfur dioxide（SO2） preconditioning on SO2/aspartate amino transferase（AAT） pathway in rats with myocardial ischemia-reperfusion injury.Methods Twenty-four healthy male Wistar rats of clean grade were randomly divided into 3 groups：sham operation group,ischemia-reperfusion group（I/R group） and SO2 preconditioning group（I/R＋S group）.Myocardial ischemia-reperfusion models were made by ligating the left coronary artery for 30 min and reperfusing for 120 min.Myocardial enzymes creatine kinase（CK） and lactate dehydrogenase（LDH） levels in plasma of rats were detected by automatic biochemical detector.High performance liquid chromatography was used to measure SO2 content in myocardial tissue.AAT1 and AAT2 protein expressions in myocardial tissue were detected by Western blot method.Results Plasma CK and LDH levels in I/R group and I/R＋S group increased significantly compared with those in sham operation group（P0.01,0.05）,but plasma CK and LDH levels in I/R＋S group were significantly lower than those in I/R group（Pa0.05）.Compared with sham operation group,the level of SO2 in myocardial tissue reduced significantly in I/R group（P0.05）,while increased significantly in I/R＋S group compared with that in I/R group（P0.01）,but the difference between I/R＋S group and sham operation group did not significant.The AAT1 protein expression in myocardial tissue in I/R group decreased significantly compared with that in sham operation group（P0.01）,while increased significantly in I/R＋S group compared with that in I/R group（P0.01）,but the difference between I/R＋S group and sham operation group did not significant.The AAT2 expression among the 3 groups had no significant difference（P0.05）.Conclusions SO2 pretreatment can reduce the plasma enzyme and exert myocardial protection.SO2/AAT pathway in myocardial tissue of rat is decreased in ischemia-reperfusion rat,while it is up-regulated with SO2 preconditioning.

关 键 词：二氧化硫 天门冬氨酸氨基转移酶 缺血再灌注 预处理 大鼠 

分 类 号：R541[医药卫生—心血管疾病]