细菌L型的医院感染及其质粒谱分析研究  

Study on the L form Bacteria Nosocomial Infection with Plasmid Profile Analysis

作　　者：陈端[1] 单斌[1] 陈德昌[1] 张秋霞[1] 骈淮燕[1] 谢琼[1] 

机构地区：[1]昆明医学院第一附属医院

出　　处：《中华医院感染学杂志》1999年第3期132-134,共3页Chinese Journal of Nosocomiology

基　　金：云南省科委应用基础研究资金资助

摘　　要：目的为了全面防止医院感染，我们对细菌Ｌ型的医院感染进行了研究。方法对医院内环境中８００份标本及医院感染患者９１８份标本分离出的金黄色葡萄球菌Ｌ型、大肠埃希氏菌Ｌ型及铜绿假单胞菌Ｌ型进行了质粒谱分析和药敏试验。结果本院环境及医院感染患者细菌Ｌ型总检出率分别为１９．０％和１６．７％。不同来源的３种细菌Ｌ型的多重耐药性无显著性差异。不同来源３种细菌Ｌ型的同源性比例均较高，分别为９０．３％、７５．０％、８４．２％。OBJECTIVE In order to prevent nosocomial infection, the L form bacteria were studied. METHODS 800 samples carried out from the hospital envionment and 918 samples from the nosocomial infected patients. Three strains of L forms (Staphyoeocaus aureus L form, Eschherichia colo L from, Pandomonos L form). were isolated and identified. RESULTS The general culture positive rates of 3 strains of L form bacteria of the hospital environment and the nosocomial patients were 19.0% and 16.7% respectiveoy. The multiple antbiotic resistance tests of the three strains from two different origins were of no significante difference (P>0.01). The homology ratios of the three strains of L form bacteria from two different origins were ruther high ie, 90.3%, 75.0% and 84.2% respectively. CONCLUSIONS The pathogenic organisms of the nosocomial infected patients by L form bacteria may be contamincded from the hospital environment.

关 键 词：细菌L型 医院感染 质粒谱 同源性分析 多重耐药性 

分 类 号：R378[医药卫生—病原生物学] R969.4[医药卫生—基础医学]