半夏组培苗丛枝菌根侵染过程研究  

Infection progress of arbuscular mycorrhizae on tissue-cultured plantlets of Pinellia ternata

作　　者：沈雪莲[1] 郭巧生[1] 刘作易 朱国胜 刘永翔 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095 [2]贵州省现代中药材研究所,贵州贵阳550006

出　　处：《中国中药杂志》2011年第2期93-96,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30070922);国家科技支撑计划项目(2009BAI74B01-1)

摘　　要：目的:探索半夏组培苗丛枝菌根菌侵染过程与特点。方法:将摩西球囊霉接种于半夏无菌组培苗,于不同时间取样,通过染色镜检法观察丛枝菌根形成过程。结果与结论:接种10 d时菌丝开始侵入半夏根表皮;15 d时大量胞内菌丝形成,也出现少量的胞间菌丝;20 d后菌丝开始形成丛枝,并出现少量根上菌丝;接种25 d后大量菌丝分叉为丛枝,以Arum型丛枝为主;30 d时一些丛枝开始消解,有泡囊的产生;35 d后半夏根部出现大量根外孢子;接种后40 d,串生泡囊形成,且部分泡囊开始收缩衰老。半夏切片结果表明,AM真菌菌丝逐渐生长在根部细胞间隙生长,然后在皮层细胞内进行分叉发育,最终形成丛枝充满整个细胞腔。整个实验表明半夏能迅速与丛枝菌根相互识别,形成共生体。Objective： To study the Arbuscular mycorrhizal（AM） formation progress and infection characteristics between tissue culture plantlets of Pinellia ternata and Glomus mosseae.Method： The tissue culture plantlets of P.ternata were inoculated with G.mosseae,the formation of AM were sampled and observed with microscopy by staining.Result and Conclusion： The hyphae of G.mosseae began to penetrate the root epidermis after 10 days of inoculation.Lots of intracellular hyphae formed in cortex cells at the 15th day.Arbuscules started to form and there were some hyphae on the root at the 20th day.At the 25th day,many arbuscules formed and most as Arum type.Some arbuscles started to disintegrate at the 30th day,and a few of vesicles occurred.Lots of spores formed after 35 days.At the 40th day,some vesicles began to decline.The hand section showed that the intercellular hyphae gradually formed in intercellular space,and the hyphae branched in cortex cells and occupied most cell lumen finally.It is expounded that P.ternata and G.mosseae could recognize each other quickly and form a symbiont system.

关 键 词：半夏 组培苗 摩西球囊霉 侵染 丛枝菌根形成 

分 类 号：S567.239[农业科学—中草药栽培]