融斑通脉颗粒干预动脉粥样硬化氧化应激作用的实验研究  

Study of Rongban Tongmai granule on anti-oxidant stress in atherosclerosis

作　　者：林成仁[1] 任建勋[1] 王敏[1] 马雪瑛[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中国中药杂志》2011年第2期195-199,共5页China Journal of Chinese Materia Medica

基　　金："国家重大新药创制"科技重大专项(2009ZX09502-017;2009ZX09303-003)

摘　　要：目的:观察家兔动脉粥样硬化(AS)过程氧化应激的状态,以及融斑通脉颗粒的干预作用。方法:将家兔分为正常对照组和高脂造模组,高脂饲料喂养至4周,将高脂造模组家兔分为高脂对照组、舒降之组和融斑通脉颗粒高、中、低剂量组,连续给药8周。于实验前、给药前、给药4,8周时测定血清中NO,ox-LDL,LP(a),SOD,MDA水平,以及给药8周时测定肝组织MDA,GSH,NO含量和SOD,GSH-Px活性,并取主动脉进行病理学检查。结果:高脂饲料喂养12周后,与正常对照组比较,高脂对照组家兔主动脉内膜明显增厚,形成明显的粥样斑块,血清MDA,LP(a)含量均明显增加(P<0.001),SOD活性及NO含量均明显降低(P<0.01,P<0.001);同时第12周后肝脏MDA含量明显高于正常对照组(P<0.01),SOD活性,GSH和NO含量则均显著降低(P<0.01,P<0.001)。融斑通脉颗粒干预8周后家兔主动脉粥样斑块病变程度明显减轻,血清中MDA,LP(a)含量明显降低(P<0.05),SOD活性和NO含量明显上升(P<0.05);同时肝脏中SOD活性、GSH和NO含量明显升高(P<0.05),MDA含量明显降低(P<0.05)。结论:抗氧化应激、纠正氧化-还原状态失衡可能是融斑通脉颗粒防治AS的机制之一。Objective： To.observe the therapeutic effects of Ronghan Tongmai granule on oxidative stress in atherosclerotic rabbits. Method： The experimental rabbits were randomly divided into control group and hyperlipidemie group. The model of experimental atheroscleresis was prepared by feeding high cholesterol and lipid diet for weeks. After 4 weeks, hyperlipidemic rabbits were randomly divided into five groups, model group, high, medium and low doses of Rongban Tongmai granule and Shujiangzhi group, taking medicine for 8 weeks. The level of NO, ox-LDL, LP（a）, SOD and MDA in the serum was measured before experiment, after treatment, treated for 4 weeks and for 8 weeks. After treated for 8 weeks, the level of MDA, GSH and NO, as well as activity of SOD and GSH- Px in the liver of rabbits was determined, meanwhile, the pathologic morphology of aortas was observed by light microscope. Result： Compared with control group, aortic intima of rabbits in model group had obviously thickened and developed atherosclerotic plaque. The serum level of MDA and LP（a） in model group had increased（P 〈 0, 01 ） at 12 weeks after feeding high cholesterol and hpid diet, but the activity of SOD and level of NO were decreased （ P 〈 0.01, P 〈 0, 001 ）. At the same time, the level of MDA in the liver had been elevated （ P 〈 0.01 ）, but the activity of SOD, GSH and NO was decreased （ P 〈 0.01, P 〈 0.001 ） in 12 weeks. Rongban Tongmai granule could inhibit atherosclerotic lesion in aorta, decrease the level of MDA and LP（a） （P 〈0.05） , inerease the activity of SOD and NO（P 〈0.05） after treated for 8 weeks in serum, moreover, the activity of SOD, GSH and NO content in the liver were increased （ P 〈 0.05 ）, as well as MDA was decreased （ P 〈 0.05 ）. Condusion： Rongban Tongmai granule can prevent atherosclerosis by antioxidative stress and correcting unbalance of redox.

关 键 词：融斑通脉颗粒 动脉粥样硬化 脂质代谢 氧化应激 

分 类 号：R285.5[医药卫生—中药学]