水环境中微生物PCR检测灵敏度的研究  

Study on Sensitivity of PCR Method for Detection of Microorganisms in Water

作　　者：王占朝[1] 刘文君[1] 

机构地区：[1]清华大学环境科学与工程系,北京100084

出　　处：《中国给水排水》2011年第2期91-94,共4页China Water & Wastewater

摘　　要：以大肠杆菌纯培养物和清华大学湖水以及自来水中的微生物为研究对象,研究了不同水环境中微生物PCR检测的检出限,结果表明:大肠杆菌JM109纯培养物的PCR检出限为2.6×103 CFU/mL,湖水的PCR检出限为1.2×103 CFU/mL,自来水中微生物的PCR检出限为2.3×103 CFU/mL。湖水和自来水中的检出限均低于大肠杆菌纯培养物。Pure culture of E. coli and microorganisms in lake water and tap water from Tsinghua University were used to investigate the detection limits of microorganisms in different water environments by PCR. The results show that the detection limit of E. coli JM109 is 2.6 ~ 103 CFU/mL, and the detec- tion limits of microorganisms in lake water and tap water are 1.2 ×10^3 CFU/mL and 2.3 ×10^3CFU/mL respectively. The detection limits of microorganisms in lake water and tap water are less than that of pure culture of E. coli.

关 键 词：微生物 PCR 灵敏度 

分 类 号：R123.1[医药卫生—环境卫生学]