HPLC法测定导赤丸中黄芩苷和大黄素的含量  

Content Determination of Baicalin and Emodin in Daochi Pills by HPLC

作　　者：吴卓[1] 郭伟英[1] 

机构地区：[1]辽宁医学院药学院,锦州市121001

出　　处：《中国药房》2011年第4期373-375,共3页China Pharmacy

摘　　要：目的:建立测定导赤丸中2种有效成分黄芩苷和大黄素的含量的方法。方法:采用高效液相色谱法。色谱柱为Chroma-trex C18柱;测定黄芩苷的流动相为甲醇-水(加入磷酸调pH=3.5,43:57),检测波长为280nm,流速为1.0mL·min-1,柱温为30℃;测定大黄素的流动相为甲醇-水(加入冰醋酸调pH=2.0,80:20),检测波长为245nm,流速为1.0mL·min-1,柱温为25℃。结果:黄芩苷和大黄素的检测浓度分别在0.626～1.044mg·mL-1(r=0.9996)和5～25μg·mL-1(r=0.9998)范围内线性关系良好;平均回收率(n=9)分别为100.18%(RSD=0.75%)和99.30%(RSD=1.5%)。结论:本方法操作简便、准确度高、专属性强,适用于导赤丸的质量控制。OBJECTIVE：To establish the method for the content determination of baicalin and emodin in Daochi pills.METHODS：HPLC was adopted with chromatrex C18 as column.The mobile phase for baicalin determination consisted of methanol-water-phosphoric acid（43：57） with detection wavelength at 280 nm.The flow rate was set at 1.0 mL·min-1 and the column temperature was 30 ℃.The mobile phase for emodin determination was methanol-water-glacial acetic acid（80：20） with detection wavelength at 245 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 25 ℃.RESULTS：There was good linearity between the peak area and concentration within the ranges of 0.626～1.044 mg·mL-1（r=0.999 6） for baicalin and 5～25 μg·mL-1（r=0.999 8） for emodin,respectively.The average recoveries of baicalin and emodin were 100.18%（RSD=0.75%）and 99.30%（RSD=1.5%）,respectively（n=9）.CONCLUSION：The method is simple,accurate and specific for quality control of Daochi pills.

关 键 词：导赤丸 黄芩苷 大黄素 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]