东南太平洋智利竹鱼RAPD遗传多样性研究  

Genetic diversity of Chile jack mackerel (Trachurus murphyi) in the southeast Pacific by RAPD analysis

作　　者：张伟[1] 张敏[1,2,3] 邹晓荣[1,2,3] 许强华[1,2,3] 谢峰[4] 吴昔磊[4] 

机构地区：[1]上海海洋大学海洋科学学院,上海201306 [2]上海海洋大学大洋渔业资源可持续开发省部共建教育部重点实验室,上海201306 [3]上海海洋大学大洋生物资源开发和利用上海市高校重点实验室,上海201306 [4]上海开创远洋渔业有限公司,上海200090

出　　处：《上海海洋大学学报》2011年第1期22-26,共5页Journal of Shanghai Ocean University

基　　金：上海市重点学科建设项目(S30702);大洋生物资源开发和利用上海市高校重点实验室(上海海洋大学)开放基金(S30702)

摘　　要：根据2009年7-12月我国大型拖网渔船在东南太平洋海域(34°S～37°S、90°W～110°W)作业期间采集的样本,选取17个站点146尾智利竹鱼肌肉样本进行随机扩增多态性DNA分析。结果显示扩增条带中共检测到52个位点,其中多态性位点为51个,多态位点占所有扩增位点的98.08%。群体的Shannon’s多样性指数为0.520 2±0.181 3,Nei’s基因多态性指数为0.350 0±0.144 0,种群间遗传分化指数GST为0.031 1。结果表明该海域智利竹筴鱼群体的遗传多态性较高,但群体间不存在显著的遗传分化。According to the Chile jack mackerel（Trachurus murphyi） samples in the southeast Pacific Ocean（34°S ~37°S,90°W-110°W） collected by Chinese trawler fishing vessel during July and December 2009,146 muscle samples collected from17 sites were used for population genetic structure identification by Random Amplified Polymorphic DNA（RAPD） analysis.52 loci were detected and 51 of these loci were polymorphic,which accounted for 98.08% of the total loci.The Shannon＇s index was 0.5202±0.1813,the Nei＇s genetic diversity index was 0.3500±0.1440,and the GSTvalues was 0.0311.The results indicated that the genetic diversity of the Trachurus murphyi was comparably high,and genetic differentiation was not significant among populations.

关 键 词：智利竹鱼 种群遗传结构 RAPD 东南太平洋 

分 类 号：S917[农业科学—水产科学]