四藤方对Bel-7402细胞增殖和凋亡作用研究  

Effects of Siteng Fang on Proliferation and Apoptosis in Bel-7402 Cells in vitro

作　　者：沈克平[1] 胡兵[1] 刘威[1] 

机构地区：[1]上海中医药大学附属龙华医院肿瘤五科,上海200032

出　　处：《中国实验方剂学杂志》2011年第3期120-123,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(30873258);上海市基础研究重点项目(09JC1413600);上海市浦东新区中医领军型人才项目(PWZ2008-20-104);上海市级医院临床科研资源共享平台(SHDC12007206)

摘　　要：目的:观察清热解毒中药-四藤方对肝癌细胞Bel-7402增殖和凋亡的影响。方法:不同浓度四藤方处理人肝癌细胞Bel-7402、人肝细胞HL-7702,WST-8法检测细胞增殖,流式细胞术检测细胞凋亡,Hoechst 33258染色检测细胞凋亡形态变化,Western Blot法检测半胱天冬酶-3(caspase-3)活化。结果:与对照组比较,四藤方对人肝细胞HL-7702增殖无明显影响;100～400μg.mL-1四藤方作用24 h可显著抑制肝癌Bel-7402细胞增殖,促使细胞Bel-7402凋亡,呈现细胞凋亡形态,并可活化Bel-7402细胞caspase-3。结论:四藤方可以抑制Bel-7402细胞增殖,促Bel-7402细胞凋亡,并可能与活化caspase-3相关。Objective：To observe the effects of Siteng Fang （STF）, a Chinese herb formula, on proliferation and apoptosis in hepatocarcinoma Bel-7402 cells in vitro. Method： Human hepatocarcinoma Bel-7402 cells and human normal liver HL-7702 cells were treated with different dose of STF. Cell proliferation was detected with CCK-8 （ cell counting kit-8, CCK-8） assay; cell apoptosis was detected by flow cytometry. Apoptotic morphology was visualized by hoechst 33258 staining, and activation of caspase-3 was detected by western blot assay. Result： STF showed no obvious cytotoxicity against normal liver HL-7702 cells. Upon treatment with 100400 mg- L-1 STF for 24 h, proliferation of Bel-7402 cells was significantly inhibited. 100-400 mg· L^-1 STF also induced apoptosis in Bel-7402 cells. In addition, caspase-3 was activated after 100-400 mg. L^-1STF treatment. Conclusion： STF may inhibit cell proliferation, and induce apoptosis in Bel-7402 hepatocarcinoma cells. Activation of caspase-3 may be involved in the process.

关 键 词：肝癌细胞 四藤方 增殖 凋亡 半胱天冬酶-3 

分 类 号：R285.5[医药卫生—中药学]