复方六月青对肝星状细胞增殖和细胞外基质的影响  

Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells

作　　者：张士军[1] 张志伟[2] 陈兆霓[1] 焦杨[1] 李勇文[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021 [2]广西中医学院附属瑞康医院,南宁530011

出　　处：《中国实验方剂学杂志》2011年第3期135-138,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与技术开发计划项目(桂科攻0322024-5E);广西研究生教育创新计划资助项目(2006105981007D14)

摘　　要：目的:研究复方六月青(CLYQ)对肝星状细胞(HSC)增殖和细胞外基质(ECM)的影响。方法:采用四甲基噻唑蓝(MTT)法检测CLYQ对HSC的毒性。采用酶联免疫吸附实验(ELISA)法检测HSC上清液羟脯氨酸(Hyp)、透明质酸(HA)和金属蛋白酶组织抑制因子-1(TIMP-1)。结果:CLYQ体外对HSC的TC50=6.191 g.L-1,TC0=3.504 g.L-1,CLYQ抑制HSC的增殖。CLYQ各剂量组均可显著降低HSC细胞上清液Hyp,HA,TIMP-1含量(P<0.05,P<0.01),呈现量效反应关系。结论:CLYQ通过抑制HSC的增殖,从而使ECM合成减少,肝纤维化减轻。Objective：To study the influence of CLYQ on proliferation and extracellular matrix （ECM） of hepatic stellate cells（HSC）. Method： Cytotoxicity caused by CLYQ was detected by MTT assay. The contents of hydroxyproline （Hyp）, hyaluronic acid （HA） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in HSC cell supernate were detected by enzyme linked immunoabsorbent assay （ELISA）. Resuit：TC50 is 6. 191 g·L ^-1, and TC0 is 3. 504 g. L^-1. CLYQ could inhibit the proliferation of HSC. The various concentration group of CLYQ could degrade significantly the contents of Hyp, HA and TIMP-1 in cell supernate （P 〈0.05, P 〈0. 01 ） , presenting the dose-effect relationship. Conelusion：CLYQ can inhibit the proliferation of HSC, so that reducing the synthesis of ECM and hepatic fibrosis.

关 键 词：复方六月青 肝纤维化 肝星状细胞 细胞增殖 细胞外基质 

分 类 号：R285.5[医药卫生—中药学]