生姜对局灶性脑缺血大鼠海马神经细胞凋亡及相关蛋白表达的影响  

Effects of Zingiberis Rhizoma Recens on Neuronal Apoptosis and Related Prote in Expression in Focal Cerebral Ischemia-Reperfusion in Rats

作　　者：贾士奇[1] 王军[1] 张红霞[2] 黄霞[1] 孙为[1] 傅蔓华[1] 

机构地区：[1]河南省中医药研究院药理实验室,郑州450004 [2]信阳职业技术学院药理教研室,河南信阳464001

出　　处：《中国实验方剂学杂志》2011年第3期163-166,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究生姜对局灶性脑缺血再灌注大鼠神经细胞凋亡及相关凋亡蛋白表达的影响。方法:SD雄性大鼠随机分为假手术组、模型对照组、尼莫地平组、生姜水提物高、中和低剂量组。采用线拴法造成大鼠局灶性脑缺血再灌注损伤模型(MCAO),分别于手术前24 h、术前1 h和再灌注后12 h ig给药,共3次,大鼠于缺血2 h再灌注24 h后,快速冰冻取脑切片固定。末端脱氧核糖核酸介导生物素化脱氧尿嘧啶缺口末端标记(TUNEL)法测定海马凋亡神经元,免疫组织化学方法研究海马神经元半胱氨酸天冬氨酸蛋白酶3(caspase-3),抗凋亡基因B细胞淋巴瘤/白血病-2(Bcl-2)和促凋亡基因(Bax)基因表达。结果:与假手术组比较,MCAO大鼠海马凋亡细胞明显增加,caspase-3和Bax基因表达增强,Bcl-2/Bax比值显著降低;生姜能明显降低MCAO大鼠海马TUNEL,caspase-3,Bax和Bcl-2阳性细胞数,Bcl-2/Bax比值显著升高。结论:生姜水提物对脑缺血及缺血再灌注损伤具有一定的保护作用,其作用机制可能与抑制神经细胞凋亡相关蛋白有关。Objective： To investigate the effect of Zingiberis Rhizoma Recens on neuron apoptosis in cerebral ischemia-reperfusion injury of rats. Method：Male SD rats were randomly allocated into six groups： sham- operation group, model contral group, nimodipine group, aqueous extract of Zingiberis Rhizoma Recens groups with high, medium or low dose groups. The model of focal cerebral ischemia-reperfusion （MCAO） in rat was produced by Zea Longa＇s thread method. The brains were taken out on the ice for paraffin processing at 24 hours after ischemia-reperfusion. Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling （TUNEL staining） was used to assay neuron apoptosis in cerebral cortex and hippocampus. The protein expressions of caspase-3 （ cysteine-containing aspartate-specific protease）, Bcl-2 （ B cell lymphoma/leukemia-2 ） and Bax （ Bcl-2 accociated X protein） in hippocampus were detected by immunohistochemistry method. Result： Compared with sham operation group, TUNEL staining positive cells and caspase-3, Bcl-2 and Bax immunoreative neurons in cortex and hippocampal tissue increased significantly, the ratio of Bcl-2/Bax decreased in MCAO rat model control group. Aqueous extract of Zingiberis Rhizoma Recens decreased TUNEL positive cells and caspase-3, Bcl-2 and Bax immunoreative neurons in hippocampal tissue significantly and increased the ratio of Bcl-2/Bax markedly, comparedwith MCAO rat model control group. Conclusion： effects on cerebral ischemia-reperfusion. Apoptosis Aqueous extract of Zingiberis Rhizoma Recens has protective signal relative proteins may be involved in the protection.

关 键 词：生姜 脑缺血再灌注 细胞凋亡 凋亡相关蛋白 

分 类 号：R285.5[医药卫生—中药学]