舒胃汤对功能性消化不良大鼠Cajal间质细胞、胃动素表达的影响  

Effect of Shuweitang on Expression of Interstitial Cells of Cajal and Motilin in Functional Dyspepsia Rats

作　　者：胡淑娟[1] 王小娟[1] 郭璇[1] 肖麟[1] 谭华梁[1] 

机构地区：[1]湖南中医药大学第一附属医院消化内科,长沙410007

出　　处：《中国实验方剂学杂志》2011年第3期170-173,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省卫生厅中医药专项基金(2008025);长沙市科技局计划(K10001044-41)

摘　　要：目的:观察舒胃汤对功能性消化不良(functional dyspepsia,FD)大鼠c-Kit阳性Cajal间质细胞(interstitial cells ofcajal,ICC)、胃动素(Motilin,MTL)表达的影响,探讨其可能的作用机制。方法:将大鼠随机分成对照组、模型组、舒胃汤高剂、舒胃汤低剂量组、多潘立酮组、木香顺气组,采用夹尾刺激法[1]建立FD大鼠模型,连续给药14 d后,采用免疫组化分析法检测胃窦MTL和c-Kit阳性ICC表达,以酚红法观察胃排空。结果:与对照组相比,模型组胃排空延迟、胃窦MTL和c-Kit阳性ICC表达显著降低(均P<0.05);与模型组相比,舒胃汤高剂量组、多潘立酮组及木香顺气组胃排空、MTL及c-Kit阳性ICC表达显著升高(均P<0.05),而舒胃汤低剂量组与模型组相比无显著性差异。结论:舒胃汤高剂组可以明显增加肝胃不和型FD大鼠饮水、食量,促进胃窦ICC和MTL表达,加快胃排空;上调ICC和MTL表达、提高大鼠胃排空率是舒胃汤治疗功能性消化不良可能的作用机制之一。Objective ： To observe the effects and mechanism of Shuweitang on expression of interstitial cells of cajal （ICC） and motilin （MTL） in functional dyspepsia （FD） rats. Method ： FD model was induced by moderate enraging the rats＇ tail. All rats were divided randomly into 6 groups： control group, model group, high and low dosages of Shuweitang groups, domperidone and Muxiangshunqi groups. Drugs were administrated for 14 days. ICC and MTL were determined by immunohistochemical method. And gastric emptying was observed with phenolsulfonphthalein. Result：Compared with the control group, gastric emptying and the expression of ICC and MTL in the model groups were obviously decreased （P 〈 0. 05）. Compared with model group, gastric emptying, MTL and ICC increased significantly in high-dose Shuweitang, Muxiangshunqi and domperidone groups （P 〈 0. 05 ）. Low dose of Shuweitang showed no significant differences（ P 〉 0. 05 ）. Conclusion ： High dose of Shuweitang may increase diet and water intake, promote ICC and MTL expression in FD rats, and accelerate gastric emptying.

关 键 词：功能性消化不良 大鼠 舒胃汤 CAJAL间质细胞 胃动素 

分 类 号：R285.5[医药卫生—中药学]