柴胡疏肝散对卒中后抑郁大鼠海马组织Bcl-2,Bax蛋白表达的影响  

Influence of Bupleurum Liver-Coursing Powder on Expression of Bax and Bcl-2 Proteins in Rats with Post-stroke Depression

作　　者：樊蔚虹[1] 姚建平[1] 赵文景[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2011年第3期181-183,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省中医管理局继承与创新专项(HNZY0702-1);郑州市科技攻关项目(10PTGS485-5)

摘　　要：目的:观察卒中后抑郁症(PSD)模型大鼠海马神经元凋亡相关基因表达的变化及柴胡疏肝散的干预作用,从细胞分子水平探索柴胡疏肝散治疗机制。方法:健康雄性SD大鼠100只,随机分为3组,即柴胡疏肝散治疗组、PSD模型组和正常组。除正常组外,其余2组首先线栓法制备局灶性脑缺血大鼠(MCAO)模型,并按longa评分标准进行评分,手术1周后开始复合制备PSD大鼠模型,同时柴胡疏肝散组(7.875 g.kg-1.d-1),ig 21 d,模型组蒸馏水ig。免疫组化法检测大鼠海马组织Bcl-2,Bax蛋白表达。结果:与正常组比较,模型组Bax蛋白阳性表达升高,Bcl-2蛋白表达下降(P<0.01);柴胡疏肝散组与模型组比较,则明显下调Bax蛋白表达(P<0.01),上调Bcl-2蛋白表达(P<0.01)。结论:柴胡疏肝散可通过下调Bax、上调Bcl-2蛋白的表达而抑制神经细胞凋亡,从而发挥对PSD的治疗作用。Objective：To observe the post-stroke depression （PSD） model of rat and hippocampal neuronal apoptosis related gene expression changes, and the intervention of Bupleurum Liver-Coursing Powder on for PSD and the molecular mechanism. Method： One hundred male SD rats were randomly divided into the following 3 groups： Bupleurum Liver-Coursing Powder group, PSD model control group and normal control group. Except the normal control group, at first the other two groups were set up middle cerebral artery occlusion （MCAO） rat model, eneurological recovery of stroke rat was assessed by Longa grades standard test. A week after surgery, we re-establish PSD composite rat model, while Bupleurum Liver-Coursing Powder group （7. 875 g·kg-1 ） was fed 21 days with the drugs, PSD model control group was fed distilled water. Immunohistochemical staining detect Bcl-2 and Bax protein expression in hippoca mpus. Result：Compared with the normal control group, Bax protein expression in PSD model group was increased, but Bcl-2 protein decreased （P 〈 0. 01 ） ; Compared with the PSD model group, Bupleurum Liver-Coursing powder group was significantly reduced in Bax protein expression （P 〈0. 01 ） and increased in Bcl- 2 protein expression （P 〈 0. 01 ）. Conclusion：Bupleurum Liver-Coursing Powder may play a role in the treatment of PSD through inhibiting the apoptosis by means of intervention with reduced Bax protein expression and increased the expression of Bcl-2 gene.

关 键 词：柴胡疏肝散 卒中后抑郁 海马神经元 基因表达 

分 类 号：R285.5[医药卫生—中药学]