灰毡毛忍冬自然群体遗传多样性的SRAP研究  

Genetic diversity of Lonicera macranthoides geographical populations revealed by SRAP markers

作　　者：陈大霞[1,2] 李隆云[1,2] 吴叶宽[1,2] 张雪[1,2] 蔡应繁[3] 

机构地区：[1]重庆市中药研究院中药种植研究所,重庆400065 [2]重庆市中药良种选育与评价工程技术研究中心,重庆400065 [3]重庆邮电大学,重庆400065

出　　处：《中草药》2011年第1期143-147,共5页Chinese Traditional and Herbal Drugs

基　　金：国家"十一五"科技支撑计划(2006BAI06A12);重庆市自然科学基金资助项目(CSTC2009BB5395)

摘　　要：目的研究灰毡毛忍冬自然群体的遗传多样性。方法以15个不同来源地的野生灰毡毛忍冬及2个花蕾型栽培品种为试材,通过SRAP分析,利用Popgene及Treeconw软件分析处理数据,UPGMA方法聚类,构建亲缘关系系统图。结果通过筛选得到24对SRAP引物,扩增出239条条带,其中210条为多态性条带,多态性条带比率为87.8%;Nei’s多样性指数He为0.239 9,Shannon多样性指数I为0.372 4;从DNA分子水平揭示出灰毡毛忍冬存在着丰富的遗传多样性。遗传相似系数在0.442 3～0.940 2。用UPGMA法得到所有样本的聚类图,可分为两大类群。结论灰毡毛忍冬有着丰富的遗传多样性,SRAP技术可有效运用于灰毡毛忍冬的遗传分析。Objective To research genetic diversity of different Lonicera macranthoides geographical populations.Methods Seventeen materials were estimated by the approach of sequence-related amplified polymorphism（SRAP）.The data of amplified bands were analyzed by Popgene 1.31 and Treeconw software.The system diagram of genetic relationship was built by UPGMA.Results The 24 pairs of SRAP primers combination employed produced a total of 239 discernable and reproducible amplified fragments.Among them there were 210 polymorphic bands.The percentage of polymorphic bands within different populations was 87.8%;Genetic diversity analysis showed that Nei’s gene diversity（He） was 0.239 9 and Shannon′s genetic diversity index（I） was 0.372 4.Basis on the DNA molecular level,the results indicated that there was abundant genetic diversity among the tested materials.Genetic similarity coefficient ranges changed from 0.442 3 to 0.940 2.The dendrogram including all samples was obtained by UPGMA.In the dendrogram,there were two cluster groups.Conclusion The genetic diversity of L.macranthoides geopraphical population in China is plentiful.SRAP markers can be effectively applied to genetic analysis in L.macranthoides populations.

关 键 词：灰毡毛忍冬 SRAP 遗传多样性 种质资源 良种繁育 

分 类 号：R284.1[医药卫生—中药学]