不同金属离子对竹黄液体发酵的影响  

Effect of different metal ions for submerged culture of Shiraia bambusicola

作　　者：项小燕[1] 郑爱芳[1] 谢翎[1] 

机构地区：[1]安庆师范学院生命科学学院,安徽安庆246011

出　　处：《中草药》2011年第1期164-166,共3页Chinese Traditional and Herbal Drugs

基　　金：安徽省教育厅自然科学研究基金(KJ2007B107);安徽省高校青年教师资助计划项目(2007jp1123);安庆师范学院科研启动项目(044-k05000000131)

摘　　要：目的研究不同质量分数的Cu2+、Fe3+、Cr3+和Ca2+对竹黄菌液体发酵产竹红菌素量和菌丝体生物量的影响。方法向培养基中添加不同质量分数的金属离子,在27℃、130 r/min下培养96 h。结果 Cr3+的质量分数为0.005%时竹红菌素及菌丝体生物量最大;Fe3+的质量分数为0.003%时菌丝体生物量最高,0.005%时竹红菌素量最高;Ca2+的质量分数为0.03%时最有利于菌丝体生长,0.05%时最能促进竹红菌素的分泌;Cu2+的质量分数为0.03%时生物量及竹红菌素量均达到最大值。结论添加金属离子后,菌丝体干质量最高达15.28 g/L,竹红菌素量最高达8.12 mg/g。Objective To study the effect of different levels of Cu2＋,Fe3＋,Cr3＋,and Ca2＋ on mycelia biomass and the hypocrellin yield by submerged culture of Shiraia bambusicola.Methods The fungis were cultured under 27 ℃ and 130 r/min for 96 h with each level of metal ions.Results The biomass and the hypocrellin yield were the highest with Cr3＋ of 0.005%;Fe3＋ of 0.003% and 0.005% contents were favorable to the biomass and the hypocrellin yield,respectively;Ca2＋ content of 0.03% was suitable for the mycelia growth and the hypocrellin yield benefited from the concentration of 0.005%;Cu2＋ content of 0.03% was added,both the biomass and the hypocrellin yield reached the best.Conclusion The highest mycelia dry weight and the hypocrellin yield could reach 15.28 g/L and 8.12 mg/g,respectively in addition of metal ion.

关 键 词：竹黄 竹红菌素 生物量 菌丝体 金属离子 

分 类 号：R282.7[医药卫生—中药学]