大豆胚尖不定芽诱导影响因子的研究  

Induction of Adventitious Buds from Embryonic Tip in Soybean

作　　者：王萍[1] 张艳君[1] 管娟娟[1] 张淑珍[2] 李文滨[2] 王罡[3] 季静[3] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]东北农业大学大豆研究所/大豆生物学教育部重点实验室,黑龙江哈尔滨150030 [3]天津大学农业与生物工程学院,天津300072

出　　处：《作物杂志》2011年第1期17-20,共4页Crops

基　　金：国家转基因生物新品种培育重大专项(2009ZX08010-013B);国家自然科学基金(30671317);大豆生物学省部共建教育部重点实验室开放课题(SB08A03)

摘　　要：以大豆胚尖为外植体,研究基因型、浸种时间和诱导时间对胚尖不定芽诱导的影响。结果表明,7个基因型大豆不定芽诱导率变化在35.0%~83.3%之间,黑农48适合于大豆胚尖不定芽再生系统;合丰35成熟种子诱导不定芽产生以浸种48h效果最好;黑农48诱导96h时不定芽诱导率达100%,诱导96h和84h大豆胚尖可形成较多的不定芽。Embryonic tips from soybean varieties were used as explants to investigate the induction of adventitious buds affected by genotype,the development stage and the time of inducing.The result showed that the shoot induction rate from adventitious buds ranged from 35.0% to 83.3% among the seven soybean genotypes.Heinong 48 showed the best in the induction among them.48h mature seed treatment was found to be more effective in adventitious bud induction in Hefeng 35.The induction rate of adventitious bud in Heinong 48 reached 100% from mature seeds treated for 96h.More adventitious buds were generated from soybean embryonic tips treated for 96h and 84h.

关 键 词：大豆 胚尖 不定芽 基因型 

分 类 号：S565.1[农业科学—作物学]