鸡血藤醇提物体外抗病毒活性研究  

Study on in vitro Antiviral Activities of Different Extracts from Caulis Spatholobi

作　　者：曾凡力[1] 程悦[2] 陈建萍[3] 符影[2] 王巧利[1] 杨得坡[2] 陈振平[1] 向阳飞[1] 王一飞[1] 王冬梅[2] 

机构地区：[1]暨南大学生物医药研究开发基地,广州510632 [2]中山大学药学院,广州510006 [3]香港大学中医药学院

出　　处：《中药新药与临床药理》2011年第1期16-20,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金.广东省自然科学联合基金(U0632010);科技部国际合作项目(2009DFA31230)

摘　　要：目的研究鸡血藤醇提物及其不同极性溶剂萃取物的体外抗柯萨奇B3病毒、单纯疱疹病毒Ⅰ型、呼吸道合胞病毒、甲型流感病毒以及乙型肝炎病毒的活性。方法鸡血藤药材60%醇提物经石油醚、乙酸乙酯、正丁醇依次萃取后浓缩制得各不同极性溶剂萃取物,观察其药物毒性、病毒引起的细胞病变效应判断药物毒性及药物抗病毒效应。结果鸡血藤醇提物无抗柯萨奇B3病毒和呼吸道合胞病毒活性,但具有抗甲型流感病毒、乙型肝炎病毒和单纯疱疹病毒Ⅰ型活性,且抗单纯疱疹病毒Ⅰ型效果显著。醇提物经初步萃取分离,得到5个不同极性溶剂萃取物,其中乙酸乙酯萃取物和水层留余物抗单纯疱疹病毒Ⅰ型活性最显著。结论鸡血藤醇提物及其乙酸乙酯萃取物和水层留余物在体外对单纯疱疹病毒Ⅰ型具有明显的抑制作用,适用于抗单纯疱疹病毒Ⅰ型治疗药物的深入研究与开发。Objective To assay the in-vitro anti-virus activities of extracts from Caulis Spatholobi （CS）on Coxsackie B3 virus （CVB3）, herpes simplex virus 1 （HSV-1）, respiratory syncytial virus （RSV）, influenza A and hepatitis B virus （HBV）. Methods The 60 % ethanol extract of CS was extracted by petrol ether, ethyl acetate and n-butanol in order to obtain the extracts with different polarity. The toxicity and antiviral actions were evaluated by observing drug cytotoxicity effect and cytopathic effect（ CPE ）. Results The 60% ethanol extract of CS had no antiviral activities against CVB3 and RSV, but inhibited infection of influenza A, HBV and HSV-1. The inhibitory effect of ethanol extract against HSV-1 infection was more significant. Among the five different polar solvents extracts obtained by liquid-liquid extraction of the ethanol extract, the ethyl acetate extract and the water residue part showed most significant anti-HSV-1 activities. Conclusion CS ethanol extract and its ethyl acetate extract and the water residue part have remarkable anti-HSV-1 activity in vitro, and can be utilized for the further research and development of anti-HSV-1 drugs.

关 键 词：鸡血藤 柯萨奇B3病毒 单纯疱疹病毒Ⅰ型 呼吸道合胞病毒 甲型流感病毒 乙型肝炎病毒 

分 类 号：R285.5[医药卫生—中药学]