化瘀止痛方有效部位对子宫腺肌病细胞增殖及Bcl-2、Bax表达的影响  

Influence of Effective Fractions of Huayu Zhitong Prescription on Proliferation and Bcl-2 and Bax Expression in Adenomyosis Cells

作　　者：关永格[1] 李坤寅[1] 曾诚[2] 王占利[1] 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2011年第1期29-33,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：教育部博士点基金(20094425110001);广州中医药大学第一临床医学院学生科技创新研究项目(yyyx0902)

摘　　要：目的观察化瘀止痛方有效部位对子宫腺肌病细胞增殖活性及凋亡基因Bcl-2、Bax的影响。方法采用胶原酶消化法培养子宫腺肌病病灶细胞,MTT法检测细胞的增殖活力,免疫细胞化学方法检测细胞Bcl-2、Bax表达的变化。结果不同浓度的化瘀止痛方醇提物、水提醇沉液、水提醇沉物及挥发油均能显著降低子宫腺肌病病灶细胞的增殖活力及Bcl-2表达,且呈浓度依赖关系(P<0.05);化瘀止痛方水提醇沉物高浓度组及挥发油高浓度组Bax的表达呈显著上升,差异有统计学意义(P<0.05),化瘀止痛方其他提取部位组Bax表达上升不明显,差异无统计学意义(P>0.05)。结论通过各提取部位组抑制子宫腺肌病病灶细胞的增殖活性,诱导其凋亡而使子宫腺肌病病灶自然缩小或消退,可能是化瘀止痛方治疗子宫腺肌病的主要机理之一,其中挥发油组作用最强。Objective To explore the influence of the active components of Huayu Zhitong Prescription（HZP）on the proliferation, Bcl-2 and Bax expression in adenomyosis cells. Methods Adenomyosis cells were digested with collagenase for primary culture. MTT was performed to observe the proliferation of adenomyosis cells, and immunocytochemical assay was applied to detect the expression of Bcl-2 and Bax. Results Alcohol extract, water extract and alcohol precipitation liquid, the sediments from the water extraction and alcohol precipitation, and the volatile oil of HZP decreased the proliferation activity and Bcl-2 expression in a dose-dependent manner （P 〈 0.05）. The high-concentration sediments from the water extraction and alcohol precipitation as well as the volatile oil increased the expression of Bax increased significantly （P 〈 0.05）. There was no statistical significance in other HZP extract groups（P 〉 0.05）. Conclusion Inhibiting cell proliferation and inducing apoptosis of adenomyosis cells may be one of the therapeutic mechanisms of Huayu Zhitong Prescription for adenomyosis, and the role of the volatile oil of Huayu Zhitong Prescription has the best effect.

关 键 词：化瘀止痛方 有效部位 子宫腺肌病 病灶细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]